For small gene panels, it is often possible and efficient to predevelop
orthogonal assays for all exons covered by the test.
Another example is the use of predeveloped
automobile models of various makes for quickly populating a downtown network.
Each FGD comprised of 8 participants which was made possible by use of predeveloped
The symbol e is taken from the predeveloped
inaccuracy mitigation measures demonstrated in Figure 10.
Additionally, they only used predeveloped
scenes, despite the technology's ability to customize the environment.
primer and probe assays were from Applied Biosystems.
Rather than trying to force the distress data into a predeveloped
climate model and then performing an isolated geostatistical analysis within each zone, a pavement behavior model was created by kriging the distress data as it naturally occurs and assessing if any geographic patterns imbedded within the distress data were captured [12-15].
assay reagents were used and were purchased from Applied Biosystems.
For analysis of IL-25 (Hs03044841_m1), IL-33 (Hs00369211_m1), TSLP (Hs00263639_m1), IFN-[gamma] (Hs00989291_m1), and GAPDH (Hs02758991_g1), predeveloped
assay reagent kits of primers and probes were purchased from TaqMan Assays (Life Technologies Korea, Seoul, Korea).
For gene expression analysis of superoxide dismutase 2 (SOD2, Hs00167309_m1), glutathione peroxidase type 1 (Gpx-1, Hs00829989_gH), and heme oxygenase 1 (HO-1 Hs00157965_m1) the predeveloped
TaqMan gene expression assays (PE Applied Biosystems) and the TaqMan Universal PCR Master Mix (PE Applied Biosystems) have been employed; eukaryotic 18S rRNA endogenous control (4319413E) was used as housekeeping (PE Applied Biosystems).
All studies were reviewed by two reviewers (P-W R, W-J Y), who extracted data from the studies with the predeveloped
forms including items such as the following: first author, publication year, sample size in each group, characteristics of participants (including age, sex, and degree of UC), diagnosis criteria of UC, details of Kangfuxinye enema and mesalamine, measured outcomes, follow-up (where available), and the number and reasons of missing participants.