polystyrene; flat-bottom, tissue culture-96-well microtiter plates (Corning Incorporated, Corning, N.Y.).
This allows medical device manufacturers to offer novel solutions using presterilized
, uncured materials for in situ cure and forming within the body.
Petri plates were presterilized
at 180 AdegC for 3 h in hot air oven.
Distilled water was used for control, and the seeds were presterilized
with sodium hypochlorite.
The prepared sterile beams were placed in containers presterilized
with 70% ethanol (Figure 1(a)).
After transfection with miR-22 lentivirus and following IR, rBMSCs were seeded onto the G-CMC scaffold that was presterilized
for 7 days and then stained with calcein AM for 15 min.
Fifty microliters of suspended cells in solution was plated onto presterilized
glass coverslips that had been coated with 0.5 mg/mL poly-D-lysine (Biomedical Technologies Inc., Stoughton, MA, USA) and 10 [micro]g/mL laminin (Invitrogen).
Cytotoxicity testing was performed by the direct contact method in which the scaffolds (7 mm diameter X 4 mm thickness discs), presterilized
with ethylene oxide gas, were seeded with L929 mouse fibroblast cells (6 X [10.sup.4] cells/scaffold) in Dulbecco's Modified Eagle's Medium supplemented with 10% (v/v) fetal calf serum and 1% penicillin/streptomycin.
From each of the test tube where MIC was performed, 1 ml was taken in a sterile Petri plate and dispensed 15 ml of melted presterilized
Mueller-Hinton media, mixed by rotating both clockwise and counterclockwise, allowed to solidify, and incubated at 35[degrees]C for overnight.
The larvae were fed daily with finely ground presterilized
dog food (Pedigree brand).
Resorting to a multivariate statistical approach allowed for an overview in describing the analytical system: when applying Discriminant Analysis (DA), in fact, pasteurized, UHT, presterilized
, and in-bottle sterilized milks were clearly separated, with 100% accuracy.