Activity towards target compounds will be established using substrate binding assays (in which the heme prosthetic group
produces spectral shifts consistent with binding of the molecules close to the heme in the active site, and indicative of their substrate-like nature) and by using analytical methods (GC- and LC-MS) to confirm that the relevant P450 BM3 mutants can oxidatively transform target herbicides to inactive derivatives.
The ANOVA also revealed a significant group difference in distance error (p = 0.01), with the prosthetic group
completing their reach closer to the target; however, the mean difference was less than 3 mm.
Heme serves as the prosthetic group
in a number of important proteins, including hemoglobin, myoglobin, catalase, peroxidase, and cytochrome c, while chlorophyll is used in chloroplast photosynthesis.