puromycin


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pu·ro·my·cin

 (pyo͝or′ə-mī′sĭn)
n.
An antibiotic, C22H29N7O5, obtained from the soil bacterium Streptomyces alboniger, that is used experimentally as an inhibitor of protein synthesis.

American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. All rights reserved.

puromycin

(ˌpjʊərəʊˈmaɪsɪn)
n
(Pharmacology) a substance with antibiotic properties, obtained from certain species of Streptomyces
Collins English Dictionary – Complete and Unabridged, 12th Edition 2014 © HarperCollins Publishers 1991, 1994, 1998, 2000, 2003, 2006, 2007, 2009, 2011, 2014
References in periodicals archive ?
Forty-eight hours after infection, cells were treated with 8 [micro]g/ml puromycin to select for a pool of puromycin-resistant clones.
Following 12 h of cell culture, puromycin (0.5 [micro]g/mL) was added to the medium and stable clones were maintained in 1 [micro]g/mL puromycin until distinct colonies appeared large enough for colony picking.
The human T-cell line, H9, was co-transfected with one plasmid containing the guide RNA sequences that has homology to the amino-terminus of the ccr5 gene and the CRISPR/Cas9 and a second plasmid containing a puromycin resistance gene.
To establish stable transfected cells (CaPan2-shEI, CaPan-2-shCTR, PANC-1-EI, and PANC-1-SCR), cells were grown in culture medium with 10 [micro]g/ml puromycin.
GFP-positive transduced hAD-MSCs measured at 72 h after transduction (Figure 4(b)) and were selected for with 2.5 [micro]g of puromycin (Figure 4(c)).
Transfected cells were monitored daily and subject to media change and antibiotic selection with puromycin (8 ug/mL) every 48 hours for 12-14 days.
The stable PPAR[alpha] silenced SW480 cells were selected by puromycin. SW480 cells (1x[10.sup.6]) were injected subcutaneously in the nude mice.
The next day, selection of transduced cells was initiated with the addition of 2 [micro]g/ml puromycin (InvivoGen, San Diego, CA, USA) into the culture medium.
The retrovirus-infected, IFN[alpha]-producing cells (IFN[alpha]-AF-MSCs) were selected due to their resistance to puromycin. For further identification, IFN[alpha]-AF-MSCs were also subjected to stem cell surface marker test (CD90, CD105, CD73, HLA-ABC, CD34, CD14, CD45, and HLA-DR) by flow cytometry.