We determined serotype by using Neufeld's Quellung
reaction and antimicrobial drug resistance by MIC testing, as previously described (10).
Confirmation and factor typing were performed by the capsular reaction test (Quellung
reaction) using specific antisera (SSI).
Serotyping was performed with the capsular Neufeld Quellung
method using some common factor antisera provided by the SSI.
(13) All isolates were confirmed again by multiplex PCR, (14) serogrouped/typed with Quellung
antisera (at Christian Medical College and Hospital, Vellore, India) obtained from Statens Serum Institute (Copenhagen, Denmark).
Serotype identification is performed by the Quellung
capsular swelling method.
All pneumococcal isolates that exhibited a positive Quellung
reaction using commercial type specific antisera obtained from Statens Serum Institute, Copenhagen, Denmark, were assigned a serotype designation.
Additionally, we determined pneumococcal serotypes by the capsular reaction test (Quellung
reaction) using specific antisera (Statens Serum Institut, Copenhagen, Denmark) .
Dos de las cepas fueron enviadas al Centro de Investigacion sobre Enfermedades Infecciosas, Instituto Nacional de Salud Publica, Cuernavaca Morelos, donde la tipificacion se realizo por medio de reaccion de Quellung
con antisueros de grupo, tipo y factor empleando sueros producidos por el Statens Seruminstitut de Dinamarca.
Los serotipos capsulares se determinaron mediante la reaccion de Quellung
, utilizando los antisueros del Statens Serum Institute de Copenhagen, Dinamarca.
Serogrouping: Serogrouping was performed for the viable isolates by: latex agglutination and the Neufeld quellung
Serotyping was performed by the Quellung
reaction using pool, group, type and factor commercial antisera (SSI Diagnostica; Statens Serum Institute, Copenhagen, Denmark).
Serotyping was performed in a reference lab (Soroka University Medical Center, Beer-Sheva, Israel) by capsular swelling observed microscopically (Quellung
reaction) after suspension in type-specific antisera.