"Neither of them saw me, and I distinctly heard Mademoiselle Stangerson repeat, as she was refolding
the paper: 'The presbytery has lost nothing of its charm, nor the garden its brightness!'--It was said in a tone at once mocking and despairing, and was followed by a burst of such nervous laughter that I think her words will never cease to sound in my ears.
She gave these answers, with her attention apparently absorbed in folding and refolding
the frilling on her nightgown.
Markleham, telegraphing the Doctor as before, and refolding
the letter, 'it would be insupportable to me to think of.'
While packing to go home, Dwight was refolding
his already neatly folded clothes to put them in his bag when he found them.
was done with descending denaturant concentration dialysis with some modification.
The company said the manuscript details how the heat-shock amplifier, arimoclomol, improves the refolding
, maturation and lysosomal activity of glucocerebrosidase (GCase), the enzyme deficient in Gaucher disease and linked to Parkinson's disease.
Since they are enclosed in inclusion bodies, purification of aggregated proteins is rather a convenient process while their refolding
is a laborious and time-consuming procedure.
"This protein starts in a folded state and goes through a global transformation, refolding
in a completely different state," said Onuchic, co-director of Rice's Center for Theoretical Biological Physics (CTBP).
The present study deals with the production, refolding
and characterization of recombinant [alpha]-amylase (AMYSBS) from Geobacillus SBS-4S.
Several nights later, while dining in what is reportedly the best restaurant in this particular city, I observed a waiter refolding
the napkin of a patron who had temporarily left the table, so this refolding
thing is obviously not just a quirk in one restaurant.
The supernatant was stepwise dialyzed at 4[degrees]C against the following refolding
buffers: (1) A (4 M urea, 0.1 mM glutathione, 0.01 mM glutathione disulfide, 1 mM EDTA, 5% (v/v) glycerol in phosphate-buffered saline [PBS], pH 8.0) for 12 h; (2) B (2 M urea, 0.1 mM glutathione, 0.01 mM glutathione disulfide, 1 mM EDTA, 5% (v/v) glycerol, 0.15 M L-arginine in PBS, pH 8.0) for 12 h; (3) C (1 M urea, 0.1 mM glutathione, 0.01 mM glutathione disulfide, 1 mM EDTA, 5% (v/v) glycerol, in PBS, pH 8.0) for 12 h; and (4) D (PBS, pH 8.0) for 12 h, twice (Shi et al.