This problem partially can be resolved by genetically engineering the mice so that they also express an easily measurable reporter gene
(e.g., [beta]-galactosidase or a fluorescent probe such as green fluorescent protein [GFP]) that is activated after Cre-mediated excision of a transcriptional stop signal.
Methods-To study flhD gene expression, the flhD promoter region was cloned in front of the open reading frame (ORF) of pUA66, contains gfpmut-2 as reporter gene
. The promoter fusion plasmid for ompR was obtained from the Open Biosystems collection.
This work describes the construction of metagenomic library from soil metagenome of high altitude region of Northwestern Himalaya using promoter less vector for entrapment of novel promoter sequences using GFP as reporter gene
The term reporter gene
is used to define a gene with a readily measurable phenotype that can be distinguished easily over a background of endogenous proteins (26).
BlueScreen HC uses Gaussia luciferase to detect the up-regulation of a key reporter gene
, GADD-45a, in response to genotoxic stress and can detect all known classes of genotoxins.
The TRAC results were compared with a luciferase reporter gene
That work eventually led to the discovery of several "resistance-associated proteins." In some of these studies, a team led by Brown used a reporter gene
in conjunction with the assay to assess the amount of fungal growth in kernels.
Fusion of the reporter gene
, GFP to each of the four Hc promoters has shown that all four strains have a functional M46 promoter.
"The higher throughput of the six-channel instrument will allow small biotech and pharmaceutical companies a cost-effective approach to medium-throughput, Aequorin-based G protein-coupled receptor (GPCR) and Luciferase-based Reporter Gene
Screening," said Andy Boorn, president of MDS Analytical Technologies.
Chloramphenicol acetyltransferase (CAT) reporter gene
expression from the Ub promoter was initially very low at day 2 post-administration but by day 35 exceeded the level of expression attained from a CMV promoter vector by 4- to 9-fold.
Further testing with luciferase reporter gene
assay revealed that each compound significantly activated the estrogen reaponse element reaulting in reporter gene
expression (P = 0.0001).
Jenkinson, et al., Development of a luciferase reporter gene
high throughput screen for 7-transmembrane receptor antagonist identification, Brit.