Fraction 20 which showed one major spot was repurified
in a small column with isocratic EtoAc/n-hexane (7: 3) and afforded 12 mg of compound 2.
Chien, "Sialic acid rescues repurified
lipopolysaccharide-induced acute renal failure via inhibiting TLR4/PKC/gp91-mediated endoplasmic reticulum stress, apoptosis, autophagy, and pyroptosis signaling," Toxicological Sciences, vol.
The concentrated [XynA.sub.MG1] was repurified
using the IMAC and then desalted as described above.
The active fraction was collected and repurified
using an analytical [C.sub.18] column (150 x 4.6 mm, 5 [micro]m) with an elution with 40% acetonitrile.
(45) These exchanges are tinged with lament for the loss for human life, but also with awe for the resilience and beauty of a seemingly repurified
Treated DNA was purified on a Zymo-Spin I column, desulfonated with 0.3 M NaOH, repurified
on a Zymo-Spin I column, and resuspended in 20 [micro]L elution buffer.
Two samples failed testing because of DNA impurities; both samples passed quality-control tests after we repurified
The attractin-containing fractions were combined, lyophilized, and repurified
by Vydac C18 RP-HPLC.
Dasani, the bottled water that Coca-Cola puts out is repurified
tap water that Coca-Cola buys from local municipal water suppliers.
Both ICPs were repurified
while all other chemicals were used as received.
After this advanced treatment, the "repurified
water," which would meet or exceed all state and federal drinking water standards, would be piped to a raw water reservoir to blend with the imported water supply.
Before each experiment, [sup.125]I-[T.sub.3] and [sup.125]I-[T.sub.4] were repurified
as previously described (3).