restriction enzyme


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restriction enzyme

n.
Any of a group of enzymes that catalyze the cleavage of DNA at specific sites to produce discrete fragments, used especially in genetic engineering. Also called restriction endonuclease.
American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. All rights reserved.

restriction enzyme

n
(Biochemistry) any of several enzymes produced by bacteria as a defence against viral infection and commonly used to cut DNA for genetic manipulation or diagnosis
Collins English Dictionary – Complete and Unabridged, 12th Edition 2014 © HarperCollins Publishers 1991, 1994, 1998, 2000, 2003, 2006, 2007, 2009, 2011, 2014

restric′tion en`zyme


n.
any of a group of enzymes that are capable of cutting DNA at specific sites along its strand.
[1960–65]
Random House Kernerman Webster's College Dictionary, © 2010 K Dictionaries Ltd. Copyright 2005, 1997, 1991 by Random House, Inc. All rights reserved.
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.restriction enzyme - any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology
endonuclease - a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes
Based on WordNet 3.0, Farlex clipart collection. © 2003-2012 Princeton University, Farlex Inc.
References in periodicals archive ?
Such as, high concordance with epidemiological relatedness, stable and reproducible DNA restriction patterns, and can be applied as a universal generic subtyping method for many different bacteria with only the choice of the restriction enzyme and electrophoresis conditions optimized for each species.
In the simple forms of genetic engineering referred to above, a major tool is the restriction enzyme. This is a bacterially derived enzyme which recognizes a fixed, usually palindromic, short (~4-8 bp) DNA sequence and provides a pair of cuts in the sugar-phosphate backbone.
By using restriction enzyme analyses of viral DNA, several studies have reported extensive intratypic genetic variability for HAdV-4 (14-16).
All target DNA fragments were amplified by Polymerase Chain Reaction (PCR) and cloned initially into the cloning plasmid pTOPO (TaKaRa, China), verified by sequencing and released by digestion with appropriate restriction enzymes, then subcloned into corresponding restriction enzyme recognition sites of the expression plasmid pcDNA3.1 (Invitrogen, USA).
Restriction Enzyme Analysis: PCR products were purified and treated with 10 U of Hinf1 enzyme restriction (Thermo Scientific, Germany) and incubated at 37 AdegC for 16 hours.
Mutations such as single nucleotide polymorphism, multiple nucleotide polymorphism, deletion, duplication and combination often cause the formation or elimination of a restriction enzyme recognition site.
A total volume of 10 to 15 uL containing the PCR product was used in restriction enzyme digestion reactions.
The method involved restriction enzyme analysis of nested PCR products obtained with primers encoding for the 65-kDa protein, which was common to all mycobacteria.
Methods, primer sequence and restriction enzyme, as well as size of fragments generated by CD14, described in Table1.
However, the results did not show a significant relationship between restriction enzyme profiles of IGS region and pathogenic variability of Fusarium wilt isolates.
Enzyme used for restriction enzyme Hae for this position is able to identify and cut at the (AG / CT) position.
The research which we are going to present here was primarily inspired by Benenson's idea of implementing simple 2-state and 2-symbol finite automata using the concept of splicing (alternately connecting complementary parts of DNA molecules after cutting them by a specific restriction enzyme) [8, 9].

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