He replaced the entire first part of his aorta and resuspended
his aortic valve to make it work again, one of the most complicated operating surgeries.
Following incubation, the cells were centrifuged, then resuspended
by gentle agitation and examined for agglutination or hemolysis.
Finally, the cells were resuspended
in RPMI 1640 medium with 10% [degrees]CTC and 10% FBS and incubated at 37 [degrees]C in 5% C[O.
Dissociated ADSCs from each well were centrifuged and resuspended
in 1 ml cryopreservation solution supplemented with Y-27632, GSH, or both Y-27632 and GSH.
The study conducted in Beijing investigated the characteristics of resuspended
road dust and its impact on the urban air concluded that resuspended
road dust from traffic is one of the major sources of aerosols in Beijing .
The red cells are then resuspended
in saline, adenine, glucose and mannitol additive solution with only a minimal volume of plasma.
After centrifuging the cell suspension three times at 800xg for 5 min, pellets were resuspended
and seeded on 100-mm culture dishes (Corning, Glendale, AZ, USA) and cultured for 6 to 8 days in Dulbecco's modified Eagle medium (DMEM; Invitrogen, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; Hyclone, Logan, UT, USA), 1 mM L-glutamine, 100 units/mL penicillin, and 0.
The white blood cells were resuspended
in PBS solution for the following experiments.
Cells were harvested at CPE of [approximately equal to] 75%, pelleted, and resuspended
in 6 mL Dulbecco's phosphate-buffered saline (DPBS); 1 mL aliquots were stored at -80[degrees]C.
Ensuring the supply of red cells resuspended
deleucotized, deleucotized platelet apheresis, fresh frozen plasma in T.
These background organisms are a concern because the bottom sediment is resuspended
, along with the FIB persisting in the sediment, during high flow conditions.
Saxitoxin derivatives are regularly dissolved in chloridric or acetic acid for better stability, so before the fixation procedure, the toxins were lyophilized and resuspended
in 25 mM MES buffer (Sigma-Aldrich, MO).