During the dry season the protozoans groups that are truly planktonic, such as oligotrichs (Spirotrichea), were predominant, whereas in the rainy season a greater input of allochthonous particulate and biological material by the rivers probably occurred, as well as the sediment resuspension
(verified by the high turbidity and low water transparency), which enriched the local biota with the entry of protozoan that tend to live on particulate surfaces, such as sessile protozoans from the Peritrichia group.
Typical proteomic workflows require enzymatic digestion, solid phase extraction, drying, and resuspension
before the reversed phase liquid chromatography-mass spectrometry (LC-MS) analysis.
can considerably increase the concentration of potential pathogens within an operating room.
"Pulverized materials from the collapse of buildings, products of combustion during and for several months after 9/11 and continual resuspension
of particulate matter during rescue, recovery and cleanup operations exposed hundreds of thousands of individuals to environmental contaminants well after 9/11," the study's authors said.
It is well known that the roadside soil contains a lot of accumulated metals, sources of which are: fuel (Pb); lubricants (Zn, Cd, Ba, Co, Mo); tyres (Ca, Zn); catalysts (Pt, Pd, Cr, Ni); protection equipment (Zn, Ca, Cr, Mn); brakes (Zn, Ca, Cu, Cr, Mn); road construction materials (Fe, Al, Mn, As); and resuspension
of road dust (Pb, Fe, Al, Mn, Zn, Cu, Ni, Mo, As) (Abu-Allaban et al.
After centrifugation and resuspension
, the cells were treated to undergo measurements of either extracellular or intracellular antigens according to their respective protocols.
The supernatant phase was discarded and the pellet formed in the bottom of 1.5mL tubes was washed twice in 70% ethanol and air-dried before resuspension
in 200 [micro]L of TE (10mM Tris-HCl pH 8.0, 1mM EDTA).
The impact of particle resuspension
on indoor environmental quality has been investigated in recent years.
Despues de verificar la identidad de las celulas cultivadas y de contarlas en camara de Neubauer -observada en el microscopio de contraste de fases-, el volumen de resuspension
de cada muestra fue criopreservado en viales a -170[grados]C.
of DNA sequencing reaction products in agarose increases sequence quality on an automated sequencer.
Un pequeno volumen de esta resuspension
se destino para efectuar, tanto los conteos celulares, como la correspondiente caracterizacion de las celulas, mientras que la gran mayoria fue depositada en placas de cultivo multipozos, con el objetivo de hacer los ensayos de actividad de SA-[beta]-gal y de cuantificacion de proteinas.
The patient and his parents were counseled regarding endoscopic true vocal fold resuspension
. They were also advised of the possible need for an open repair with laryngofissure and tracheotomy if the endoscopic approach failed to provide satisfactory results, and they provided informed consent.