reverse transcription


Also found in: Thesaurus, Medical, Acronyms, Encyclopedia, Wikipedia.

reverse transcription

n.
The process by which DNA is synthesized from an RNA template by means of the enzyme reverse transcriptase.
Mentioned in ?
References in periodicals archive ?
Characteristics of 24 patients with positive and negative results for infection with Zika virus, Brazil, 2015 Reverse transcription Mean (SD) No.
Reverse transcription was performed in a total reaction volume of 10 [micro]L at 55 [degrees]C for 30 min and then heat-inactivated at 85[degrees]C for 5 min.
Drugs that blocked viral entry or that blocked the start of reverse transcription stopped the killing.
HIV RNA extraction, reverse transcription, amplification, recombinant vector preparation, and quantitative assessment of viral replication in cell culture are steps of
Our examination of footplate fragments by reverse transcription polymerase chain reaction testing in 95 patients with otosclerosis revealed the presence of measles virus RNA in 83% of cases.
As well as being thoroughly updated and revised, three new Chapters have been added on the use of reverse transcription (RT) - polymerase chain reaction (PCR) to detect and quantify contamination of shellfish with hepatitis A virus, new procedures for the alkaline phosphates test to determine whether dairy foods were prepared with pasteurized milk and the use of PCR to detect toxigenic vibrio cholerae in foods.
Much of the work toward achievingsomatic-cell transfer in humans with LNS has been done using retroviruses, which are viruses that use RNA and synthesize it into DNA, in a process called reverse transcription.
The first step consists of cDNA synthesis using the GoScript[TM] Reverse Transcription System.
Reverse transcription PCR (RT-PCR) was conducted using a primer pair targeting nonstructural protein 1 (NSP1) of Getah virus (OneStep RT-PCR Kit, QIAGEN, Hilden, Germany) by using the RNA extracted from the blood samples (8).
Among the protocols detailed in the 21 chapters are detection and sizing of telomeric and other simple repeats by dideoxy-PRINS, brain tissue preparations for chromosomal PRINS labeling, dual-color PRINS for in situ detection of fetal cells in maternal blood, and application of reverse transcription in situ PCR in cancer analysis.
In addition to fragmentation, another big challenge posed by FFPE samples is the cross linking of RNA to other biomolecules in the tissue during the formalin fixation process that makes the RNA inaccessible to reverse transcription (RT).

Full browser ?