Individual plate counts were recorded and the mean number of revertant
colonies per plate as well as the standard deviation was calculated.
Although other published methods aim to exclude revertant
fibers from the overall analysis, (12) the currently described method generates data for every fiber identified via merosin staining.
Schneider and her associates hypothesized that fractional ablative laser treatment and topical PLLA might help a 27-year-old RDEB patient with revertant
mosaicism who presented for management of large, nonhealing erosions on her upper back and posterior neck, complicated by frequent Staphylococcus infections.
Jackson, "The molecular basis of brown, an old mouse mutation, and of an induced revertant
to wild type," Genetics, vol.
A result was considered positive if a concentration-related increase in revertant
colonies occurred and/or a reproducible biologically relevant increase in revertant
colonies for at least one concentration occurred in at least one strain with or without metabolic activation.
In all the studied strains, no increase in the number of revertant
colonies was detected, as well as no dose-dependence was observed (Table 3), suggesting the absence of mutagenic potential of 4b.
Also, the recombinant viruses used in the NYVAC transcriptional studies have not been controlled for the potential inadvertent selection of mutations during the generation of the recombinant viruses, through the construction of revertant
viruses or sequencing of the complete viral genomes, and thus the presence of additional mutations in other genes that may influence the reported results cannot be formally ruled out [38, 39].
LSC are likely to rely on physiological extra-/intra-cellular signals for support of their survival and self-renewal.
The number of revertant
colonies per his+ plate was measured after 66 h at 37[degrees]C.
TABLE 2: Number of Salmonella typhimurium TA97, TA198, TA100, TA1535, and TA102 revertant
colonies per plate in the presence of the PBN derivate following the preincubation procedure of the Salmonella/microsome assay in the absence (-) and in the presence (+) of exogenous metabolizing system from rat liver S9-mix.
Until alternative vaccines become commercially available, control of AHS will remain reliant on the use of AHSV-LAV coupled with the adoption of strategies to minimize the likelihood of natural dissemination of revertant
and reassortant vaccine-derived viruses.