After having been left for a year or two, and then rewashed
, it yields gold; and this process may be repeated even six or seven times; but the gold each time becomes less in quantity, and the intervals required (as the inhabitants say, to generate the metal) are longer.
I remember when my wife put nappies on the line in Forest Hall they often had to be rewashed
as they seemed to attract coal dust in the air like an old-fashioned fly paper.
Following this, coverslips were rewashed
, placed on a sample holder and analysed after adding ATP solution under a confocal microscope Leica TCS-SP5 II supplemented with Leica Confocal Software Version 2.00 (17).
and Spyro: Year of the Dragon have been rewashed
and spruced up in crisp HD.
Afterward, the cells were rewashed
and incubated with a diluted Alexa-Fluor 488 goat anti-rabbit secondary antibody (Eugene, OR, USA, 1:1000) for 45 minutes in the dark.
The nanoparticles were collected by 1680 xg centrifugation for 10 min, rewashed
with ethanol, and finally dispersed in 4 ml of cyclohexane.
After that, the sections were incubated with biotinylated goat anti-rabbit IgG antibodies at 37[degrees]C for 15 min, rewashed
with PBS, incubated with the streptavidin-biotin complex (SABC) at 37[degrees]C for 15min, and then stained with diaminobenzidine under a microscope.
Sections were treated with 5 [micro]g/ml proteinase K for 2 minutes at room temperature and rewashed
The retained residue was incubated for 50 min with 2 mol/L hydrochloric acid and rewashed
to pH 6.5-7.0 after centrifugation.
Freshly harvested cassava tubers were washed, peeled, cut into small pieces, rewashed
, and weighed.
Cells growing on slides were fixed in 1% formaldehyde for 30 min, permeabilized (using 1% Triton-X100 in PBS) for 10 min, and rewashed
before application of TUNEL reagents diluted to 50% with TUNEL dilution buffer.
Cells were rewashed
with fresh medium after 24 and 48 h of incubation and 100 [micro]L of MTT solution (1mg/mL) was introduced to the wells, followed by a 4 h incubation.