Cauliflower-shaped tissues were formed at the distal ends of the claspers, and cartilage associated with the rhipidion
(small cartilaginous elements located at the distal end of the clasper) was observed along clasper grooves.
In males, the length of claspers was measured and the maturity was determined by the presence of calcified claspers, which must rotate 180[degrees] relative to their normal position, as well as a freely opening rhipidion
and fully developed siphon sacs (Clark & von Schmidt 1965; Pratt 1979).
Males presenting uncalcified claspers that did not rotate easily, an unopened rhipidion
and sperm absent from the seminal vesicle were considered juveniles; whereas adults presented calcified claspers with easy rotation, an open rhipidion
and spermatozeugamata in the seminal vesicle (Pratt 1979).
Males were deemed mature if they possessed hardened claspers and the rhipidion
At this stage, claspers are lengthening and the head of the clasper (rhipidion
) is beginning to develop, whereas at 149 cm FL and greater, spermatophores appear in the ampulla epididymis.