In a one-neck round-bottom flask
(250 mL), potassium carbonate (27.61 g, 199.78 mmol) was added to a solution of catechol (1) (10 g, 90.81 mmol) in anhydrous acetone (80 mL).
5.00 g steam explosion lignin and 150 mL distilled water were placed into a 500 mL three-neck round-bottom flask
. The mixture in the flask was heated to 30[degrees]C in a water bath and was stirred mechanically.
Specifically, 10.0 g (0.042 mol) of cobalt(II) chloride hexahydrate was solubilized in 40.0 mL of distilled water in a round-bottom flask
with stirring and 30[degrees]C heating resulting in a magenta-colored solution.
A 100 mL round-bottom flask
was equipped with a magnetic stir bar and a reflux condenser.
A sample of 5.0 g turmeric in 30 mL dichloromethane was prepared in a 50 mL round-bottom flask
with a magnetic stirrer.
Into 250 mL round-bottom flask
, bisphenol-a (4.57 g, 100.0 mmol), aniline (3.65 g, 100 mmol), paraformaldehyde (2.5531 g, 400 mmol) were added.
To a 25 mL round-bottom flask
, 2-aminopropane-1,3-diol 1a (11 mmol) (Sigma-Aldrich, 98%) was added and dissolved in a 1: 1 mixture water/1,4-dioxan (10 mL).
Borane dimethylsulphide (10M solution), 5ml (50 m mol) was taken in a 200ml round-bottom flask
, provided with a rubber stoppred side arm and fitted with a condenser connected to a mercury bubbler.
Saturated aqueous sodium bicarbonate (10 mL) and diethyl ether (5 mL) were added to a three-neck, round-bottom flask
, equipped with a reflux condenser, nitrogen inlet/outlet, and dropping funnel.
A dry 250 mL round-bottom flask
equipped with reflux condenser, magnetic stirrer bar, and septa was purged with nitrogen.
The 3400 workstation can perform virtually all chemistries and reaction conditions that are possible using traditional round-bottom flask
equipment, but with computer-controlled repeatability and round-the-clock automation.
For commercial ginkgo products, one or more capsules/tablets representing a single dose of ginkgo was weighed and introduced into a 20-ml PTFE capped sample vial and extracted with 15 ml of 80% methanol using a sonicator for 60 min at room temperature followed by filtration through a Whatman # 40 filter paper into a 250-ml round-bottom flask
. The residue was returned to the flask, a second 15 ml portion of 80% methanol was added, the mixture was sonicated again for 60 min at room temperature, filtered into the same round-bottom flask
, and the residue was washed with methanol (3 x 15 ml) while on the filter.