Se, originating from Se[O.sub.2] and existing as selenous
acid, was also adsorbed at 36.2 and 31.4% by 1 and 2, respectively, and the adsorption ratios were increased to ca.
Louis, Missouri, USA), ITS (6.25 [micro]g/mL insulin, 6.25 [micro]g/ mL transferrin, 6.25 [micro]g/mL selenous
acid) (Gibco, Thermo Fisher Scientific, Monza, Italy) for 7, 14, 21, and 28 days.
Copper chloride (Cu[Cl.sub.2] anhydrous 98%), indium chloride (In[Cl.sub.3] anhydrous 98%), gallium chloride (Ga[Cl.sub.3] anhydrous 99%), and selenous
acid ([H.sub.2]Se[O.sub.3] anhydrous 98%) were used as precursors and anhydrous ethylenediamine, triethanolamine, oleylamine, polyetheramine, and oleic acid were used as organic solvents.
ITS: insulin, transferrin and selenous
acid, bovine serum albumin, linoleic acid.
Se[O.sub.2] dissolves in water to form selenous
CHLA-15, CHLA20, and CHLA-90 neuroblastoma cells were cultured in Iscove's modified Dulbecco's medium supplemented with 3 mM L-glutamine, 5 [micro]g/mL insulin, 5 [micro]g/mL transferrin, and 5 ng/mL selenous
acid (ITS Culture Supplement; Collaborative Biomedical Products, Bedford, MA) and 20% fetal bovine serum (FBS).
About 8 x [10.sup.5] cells were centrifuged into a pellet at 450g for 10 min in a 15 mL tube and cultured in chondrogenic medium, which contained low-glucose Dulbecco's modified Eagle's medium (Gibco, Invitrogen Carlsbad, CA), supplemented with 10ng/mL transforming growth factor-[beta]3 (R&D Systems), 500 ng/mL bone morphogenetic protein-2 (R&D Systems), 10-7 M dexamethasone, 50 ug/mL ascorbate2-phosphate, 40 ug/mL proline, 100 ug/mL pyruvate (Sigma-Aldrich), and 1:100 diluted ITS + Premix (6.25mg/mL insulin, 6.25 mg/mL transferrin, 6.25 mg/mL selenous
acid, 1.25 mg/mL bovine serum albumin, and 5.35 mg/mL linoleic acid) (Becton Dickinson, Franklin Lakes, NJ).
The islets 75-150 [micro]m in diameter were cultured for 24 hr in CMRL1066 medium containing 5.5 mM glucose, 10% fetal bovine serum, 100 U/mL penicillin-streptomycin, 2 mM L-glutamate, 25 mM HEPES, and ITS Premix (5 mg insulin, 5 mg transferrin, and 5 [micro]g selenous
I added 6 mL of concentrated sulfuric acid, containing 4.2% selenous
acid, and 3 mL of 30% hydrogen peroxide and heated samples 1 h at 400[degrees]C with a block digestor (model 2040, Tecator, Herndon, VA).
The remaining dried soil was kept for analyses of total nitrogen (N) with the indophenol method and phosphorus (P) with the molybdenum blue method, after digesting 250 mg of soil in a sulfuric acid and selenous
acid mixture for 1 h (Kedrowski 1983).
Louis, MO, USA), and 50 mg/ml ITS+ Premix (Becton Dickinson; 6.25 [micro]g/ml insulin, 6.25 [micro]g/ml transferrin, 6.25 ng/ml selenous
acid, 1.25 mg/ml BSA, and 5.35 mg/ml linoleic acid).
The chondrogenic medium was DMEM-LG with ITS (6.25 [micro]g/mL insulin, 6.25 [micro]g/mL transferring, and 6.25 [micro]g/mL selenous
acid) (Collaborative Research), 1mM pyruvate (Gibco), 37.5 [micro]g/mL ascorbate (WACO), 0.1 [micro]M dexamethasone (Decadran, Merck), and 0.06 ng TGF-[beta] (R&D System).