This works provides an expansion in the area of aliphatic polyimides with the objective to be used in semidry
lithium ion polymer batteries .
Then the samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred on nitrocellulose membranes (BIO-RAD Membrane, Hercules, CA, USA; 0.22 [micro]m) by semidry
transfer cell (BIO-RAD, USA).
Then, equal concentrations of protein samples were separated by electrophoresis on sodium dodecyl sulfate (SDS)-polyacrylamide gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore) with a semidry
transfer system (Bio-Rad Laboratories, USA).
It has semidry
and subhumid climates, with an annual rainfall of 850 mm, minimum temperature of 16 C and maximum of 32 C, and has a vegetation composed mostly of tropical deciduous and subdeciduous forests.
transfer unit (Hoefer Scientific, Holliston, MA) was used to transfer the separated proteins onto a PVDF membrane (Bio-Rad) at 15 V for 50 mins.
Proteins were then transferred from gel to PVDF membrane (Immobilon P; Millipore, Bedford, MA) in a semidry
electroblotting system (Bio-Rad) at 25 V for 50 min.
The separated proteins were transferred to PVDF membranes by using a Bio-Rad semidry
transfer unit (Bio-Rad, Hercules, CA, USA).
After gel electrophoresis (200 V, 30 min), proteins were transferred to nitrocellulose membranes (Amersham[TM] Protran[TM] Premium 0.2 [micro]m NC, GE Healthcare Life Sciences) via semidry
blotting (80 mA per gel).
The proteins were transferred onto the membrane by the semidry
At present, there were mainly dry, wet, and semidry
methods for the preparation of starch phosphate at home and abroad.
Protein samples obtained as described were subjected to 12% SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to a polyvinylidene difluoride membrane (Millipore, Bedford, MA, USA) using a semidry
transfer cell (Bio-Rad).