tubules were observed and analyzed in each sample, with the same instrumental and program used for the morphometric analysis (HRP/DAB).
(2) Tubular Fertility Index is the percentage of seminiferous
tubules containing germ cells.
During this process, the germ line stem cells embedded in the supporting Sertoli cells of the seminiferous
tubules divide mitotically to expand the pool of spermatogonia; after two meiotic divisions, these actions will give rise to spermatocytes and haploid spermatids.
At 109 and 110 day-old sturgeon, developing testicular tissue was observed and included the presence of seminiferous
tubules and vas deferens.
No data are available in reptiles about the localization of the transcripts of both androgen and estrogen receptors in the seminiferous
Group I, normal control rats (Figure 5(a)), shows normal spermatogonia, Sertoli cells, and Leydig cells with accumulation of spermatozoa within the seminiferous
In our previous study, we showed that seminiferous
tubule diameter was reduced and basement membrane was thickened in seminiferous
tubules and degenerated germ cells in diabetic animals .
The following measurements were performed in testis tissue: relative volume and surface area of seminiferous
epithelium, intraepithelial spaces, tubule lumen, blood vessels, interstitium, tubule surface area, and tubule diameter.
The features evaluated were the diameter of seminiferous
tubules, diameter of lumen, and width of spermatogonia layer, spermatocytes layer, and spermatid-sperm layer.
Infertility, not amenable to treatment, is the final analysis with these patients; however, some have explored intracytoplasmic sperm injection (ICSI) in rare cases of MGD with spermatogonia present.[sup.8] Testicular pathologic investigations typically reveal the presence of mature seminiferous
tubules; however, the architecture is typically disorganized with hyalinization and atrophy of the tubules.[sup.8] In a recent case series of 20 MGD patients, none have been successfully fertile.
Next, the suspension was filtered through a 150 mesh (104[micro]m) filter to collect the seminiferous
In the untreated diabetic rats, hyperglycemia severely damaged the testes morphology as well as the spermatogenic process as evidenced by the: thickness of basement membrane of the seminiferous
tubule; mitochondria alteration; abnormal spermatocyte cells displaying polymorphous nuclei, cytoplasmic vacuolization and necrosis; and disorganization and degeneration of sperm germ cells.