The research team found that the number of undifferentiated spermatogonia per seminiferous tubule
increases steadily with age until about age 11, then rise sharply.
The values of primary testis volume, absolute Cavalieri testis volume, the volume of seminiferous tubule
epithelium and lumen, the significant increase (P=0.008) in the total number of Leydig cells compared to the control group.
Diameter of seminiferous tubule
was expressed as mean +- standard deviation and the significant difference was determined using one way analysis of variance (ANOVA) followed by post Hoc Tukey test.
In control positive group 2 shows closely packed small round abnormal spermatocyte massive decrease of spermatozoa within the lumen of seminiferous tubule
, complete absence of sustentacular cells and secondary spermatocytes included cytoplasmic vacuolation (Fig.
brasiliana resulted from lesions and could be characterized as a generalized impairment of spermatogonia maturation with exfoliation of degenerative cells and loss of seminiferous tubule
cell layers (as observed in Table 2), including a loss of type A1 spermatogonia.
MNGCs are one of the most specific and common indicators of seminiferous tubule
(ST) degeneration in testicular toxicity studies.
The diameter and circumference of seminiferous tubule
for each animal was determined by measuring at least 15 tubules from multiple fields and averaging the values .
Infertility tests with testicular biopsies have generally been limited to microscopic examination by providing an assessment to the process of spermatogenesis in each seminiferous tubule
. Research on genes involved in spermatogenesis failure is important to improve the quality of testicular biopsy examination for the causes of infertility.
The study of the seminiferous tubule
stereology and the expression of PCNA and apoptosis in the rat testes during the postnatal development.
The diameters of germinal cell nuclei were calculated by measuring 20 nuclei of each cell type at cross sections of seminiferous tubule
stages, with the exception of elongated spermatids, in which the lengths of nuclei in the cells were measured.
It has been demonstrated in earlier studies that Cd exposure increased oxidative stress producing imbalance of harmones controlling the reproductive system by disrupting the hypothalamic-pituitary-testicular axis; it also increased production of apoptosis.4 Cadmium damages the male reproductive organs by causing testicular degeneration and seminiferous tubule
damage.5 Amara observed decrease in weights of testes and seminal vesicle of rats treated with Cadmium which is associated with elevated lipid per oxidation in different organs including kidney, liver and testes.6,7
Under this scoring system, epithelial maturation in seminiferous tubule
was scored from 1 to 10.