Flotation method with Sheather
's sugar solution followed by light microscopy was also used for routine parasitological detection.
A probability density function was then calculated for each metric by using a band-width determined by Sheather
and Jones's (1991) criteria.
Faecal sedimentation, Sheather
's sugar flotation and Sodium nitrate flotation techniques out of which 17 samples revealed positive for S.
Las muestras fueron procesadas por medio de dos tecnicas: directo salina-lugol, para detectar protozoarios como Giardia duodenalis; y la tecnica de Sheather
con solucion hipersaturada de azucar para detectar ooquistes de coccidios y huevos de nematodos.
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We thank Hendrik John Weimar, Barbara Maslen, and Allen Sheather
for facilitating collection of environmental samples and Samuel Yaw Aboagye for assisting with testing of collected samples.
After that procedure, samples were mixed separately in Sheather
's sucrose solution 1 : 1 (2.5 mL [K.sub.2][Cr.sub.2][O.sub.7] containing sporulated oocysts + 2.5 mL Sheather
's solution) for floatation of oocysts and collection under a coverslip for 20 minutes in 5-mL plastic tubes.
In this study, the prevalence of Cryptosporidium in Java sparrows is 13.42%, which is higher than the 4.86% prevalence in birds in Brazil by PCR , 8.1% in white Java sparrows in China by Sheather
's sugar flotation technique , and 3.22% in parrots in China by ELISA .
Fecal pellets were collected from traps and handling bags and screened for coccidian oocysts by centrifugal flotation in modified Sheather
's solution (Barnard and Upton, 1994).
Concentration techniques: [R] Formol-ether sedimentation and Sheather
's sugar floatation technique were employed for all specimens for the concentration of the parasitic ova and cysts.