Trichostatin A rescues the disrupted imprinting induced by somatic cell nuclear transfer
Table I.- Effect of different exogenous Npm or PGA on the efficiency of BMI somatic cell nuclear transfer
The enucleated egg is essential for somatic cell nuclear transfer
because the DNA has to be reprogramed for embryological development.
Epigenetic memory of active gene transcription is inherited through somatic cell nuclear transfer
. Proceedings of the National Academy of Sciences of the United States of America, v.102, n.6, p.1957-1962, Feb.
Producing primate embryonic stem cells by somatic cell nuclear transfer
. Nature 2007;450(7168):497-502.
When a flatworm is cut in half, or when an organism is cloned via somatic cell nuclear transfer
, a single organism gives rise to two distinct organisms.
NEW YORK, N.Y., November 6, 2014 -- Scientists who conducted a study comparing induced pluripotent stem (iPS) cells and embryonic stem cells created using somatic cell nuclear transfer
(SCNT) found that the cells derived from these two methods resulted in cells with highly similar gene expression and DNA methylation patterns.
Somatic cell nuclear transfer
includes complicated procedures in which the cloned embryo is cultured in vitro which is essential for successful production of transgenic cloned animals.
Scientists have instead turned to two methods to create stem cells, apart from human embryos: somatic cell nuclear transfer
(SCNT), in which genetic material from an adult cell is transferred into an empty egg cell; and induced pluripotent stem cells (iPS cells), in which adult cells are reverted back to a stem cell state by artificially turning on targeted genes.
Production of goats by somatic cell nuclear transfer
. Nat Biotechnol 1999;17(5):456-461.
The technique used by Mitalipov, Paula Amato, M.D., and their colleagues in OHSU's Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynecology, is a variation of a commonly used method called somatic cell nuclear transfer
, or SCNT.
journal Cell said the team used a technique known as somatic cell nuclear transfer
, involving the replacement of nuclei in human ova with nuclei from donor skin cells, "generating embryos that are almost an identical genetic match to the donor individual."