avium was identified with ability to ferment mannitol, sorbose
The Enterococcus strains were investigated via biochemical reaction tests including fermentation of sugars (e.g., arabinose, sorbitol, mannitol, sorbose
, and sucrose) and arginine dihydrolase.
Also, fermentation test of salicin, trehalose, sorbose
, ornithine decarboxylase, inositol, and xylose was performed .
Biochemical characterization of strain B14 Characteristic Strain B14 Colony pigmentation Creamy Motility Utilization of: + Lactose + Xylose + Maltose + Fructose + Dextrose + Galactose + Raffinose + Trihalose + Melobiose + Sucrose + L-arabinose + Mannose - Inulin + Sodium gluconate + Glycerol + Salicin - Dulcitol - Inositol - Sorbitol - Mannitol + Adonitol - Arabitol - Erythritol - a-methyl-D-glucoside - Rhamnose - Cellobiose + Melezitose + a-methyl-D-mannoside - Xylitol - D-arabinose + Citrate - Malonate - Sorbose
- ONPG hydrolysis + Esculin hydrolysis + Table 2.
Deionized water was the eluent (0.6 ml/min), and sorbose
was the internal standard.
Species characterization was carried out by carbohydrate fermentation test using 1% sugars such as sucrose, sorbose
, sorbitol, mannitol, glucose, pyruvate, inulin, ribose, melibiose, raffinose, arabinose, and arginine.
Isolates were replica plated in microtiter plates containing 180 [micro]l of phenol red broth base (BBL) supplemented with 1% sugar solutions (arabinose, mannitol, methyla-[alpha]-glucopyranoside (MGP), ribose, sorbose
, or sorbitol) and incubated at 37[degrees]C for 24 h.