Regeneration was completely arrested at 150 mg/L kanamycin and 350 mg/L streptomycin but spectinomycin
failed to arrest the regeneration.
One thousand one hundred and forty four strains of E.coli were isolated from a municipal wastewater treatment plant, cattle, or deer in the Dry Creek watershed in central Alabama and tested for resistance to ampicillin, chloramphenicol, erythromycin, neomycin, oxytetracyclin, spectinomycin
dihydrochloride, streptomycin sulfate, and tetracycline hydrochloride.
At a minimum, antimicrobial-susceptibility testing should be performed for ceftriaxone, spectinomycin
, azithromycin, and any other regimens that are used locally for gonorrhea treatment.
There is little doubt that the antibiotic resistance markers for streptomycin and spectinomycin
will be transferred to soil bacteria and to a range of animal pathogens.
Boslego's team, which includes researchers from the Walter Reed Army Institute of Research and the Children's Hospital National Medical Center in Washington, D.C., and the University of Maryland in College Park, calculates that the spectinomycin
failure rate among U.S.
resistance was observed to emerge rapidly among US service members stationed in South Korea after spectinomycin
was adopted as the primary gonorrhea treatment by the US military (following the emergence of PPNG) (32).
After the last passage the aliquots of the appropriate dilutions were plated in triplicate on the plates LB agar (LB supplemented with 15 g [L.sup.-1] of bacto-agar) with 75 mg/ml of spectinomycin
and without antibiotic to compare the titres of the cells containing the plasmid and the cells that had lost the construct.
Kirby-Bauer antibiotic testing of Aeromonas species revealed antibiotic susceptibility to amikacin, aminosidine, third-generation cephalosporins, second-generation quinolones, colistin, gentamicin, spectinomycin
, trimethoprim-sulphonamides, and tetracycline.
When necessary, 100 [micro]g/mL of spectinomycin
(Spc) (Sigma) or 4 [micro]g/mL of chloromycetin (Cm) (Sigma) were used for SS transformants, and 50 [micro]g/mL of ampicillin (Amp) (Sigma) was applied to screen the E.
The transformed cells were grown overnight at 37 [degrees]C on LB/agar plates containing 100 [micro]g/ml of spectinomycin
. A total of 120 colonies were selected for screening and plated onto LB/agar grid plates with spectinomycin
(l00ug/ml) and incubated at 37 [degrees]C overnight.
Another drug no longer available is spectinomycin
, formerly used in areas with a high prevalence of quinolone-resistant N.
distribution of spectinomycin
(Trobicin[R]) in November 2005; remaining inventory will expire in May 2006.