Control group Table 3: The number of different testis cell types (x[10.sup.6]) Groups Spermatogonia A Spermatogonia B Sertoli cell Control 0.83[+ or -]0.06 0.16[+ or -]0.07 2.7[+ or -]0.2 BD1/2 0.83[+ or -]0.05 0.13[+ or -]0.05 2.5[+ or -]0.1 BD1/4 0.84[+ or -]0.05 0.18[+ or -]0.05 2.7[+ or -]0.3 Groups Primary Round Long spermatocyte spermatid spermatid
Control 12.1[+ or -]0.98 31.8[+ or -]2.49 30.9[+ or -]3.02 BD1/2 12.0[+ or -]0.80 31.0[+ or -]2.18 30.6[+ or -]2.66 BD1/4 12.4[+ or -]0.92 32.2[+ or -]2.20 32.1[+ or -]2.65 Groups Leydig cell Control 2.2[+ or -]0.02 BD1/2 2.5[+ or -]0.15 (**) BD1/4 2.1[+ or -]0.03 The values (n=5) are presented as mean[+ or -]SD.
Prolonged dosing resulted in continued spermatocyte degeneration as well as maturation depletion of the spermatid
population, leaving tubules containing only sustentacular cells, spermatogonia and preleptotene spermatocytes.
The Johnsen score (13) Score Level of spermatogenesis 1 No seminiferous epithelial cells, tubular sclerosis 2 No germ cells, Sertoli cells only 3 Spermatogonia only 4 Few spermatocytes, arrest of spermatogenesis at the primary spermatocyte stage 5 Many spermatocytes 6 Few early spermatids
, arrest of spermatogenesis at the spermatid
stage 7 No late spermatids
, many early spermatids
8 Less than five spermatozoa per tubule 9 Slightly impaired spermatogenesis 10 Full spermatogenesis Table 2.
In the evaluation of the spermatogenesis stages, 100 sections of the seminiferous tubules of animals' testis in puberty and sexual maturity, were classified into stages of I-VI (presence of two generations of spermatids
), VII-VIII (mature spermatids
present in lumen), IV-XII (presence of a spermatid
generation) XIV (presence of secondary spermatocytes) (Ferreira, Lison, & Valeri, 1967).
Male germ cells used in this study derived from two time points during development: pachytene spermatocyte (PS) acquired during prophase of meiosis I, and round spermatid
(RS) acquired after completing meiosis.
One important stage in spermatogenesis is the meiotic division that occurs when the primary spermatocyte develops into spermatid
. Meiosis is the process of cell division through double cleavage and the reduction of the number of chromosomes.
This method can better determine the size and position of spermatid
PSC = primary spermatocyte; SSC = secondary spermatocyte; ST = spermatid
; SPZ = spermatozoa; SZ = spermatozeugmata.
Nucleus of Sertoli cells and germ cells (spermatogonia type A, pachytene primary spermatocyte and round spermatid
) were counted in 20 seminiferous tubules per rat (n = 10 animals [group.sup.-1]) at stage VII of spermatogenesis, under a light microscope (Leica DMLS), at x 400 magnification.
FW, follicular wall; Lu, lumen; Ct, connective tissue; MS, mature spermatozoa; St, spermatid
; Spz, spermatozoa; DSz, degenerative spermatozoon.
(a) The control rabbit showing normal appearance of spermatogenic cells; spermatogonia (sg), primary spermatocyte (ps), spermatid
(sp), and Sertoli cells (sc).