The selected strains were stored in PDA slant tubes at +4[degrees]C and in sporal solution in Eppendorf tubes containing Potato Dextrose Broth (PDB) with 25% glycerol at "20[degrees]C.
After the solidification of the culture medium, one sterile disk was deposited in the center of the plate and then saturated with 10[micro]l of sporal suspension ([10.sup.6] spores/ml).
On each transfer, the slants were washed carefully with sterilized distilled water to form a sporal
Pollen and tapetum development in Ceratozamia mexicana (Cycadaceae): sporal
origin of the exinic sporopollenin in Cycads.
This recovery rate could be underestimated as sporal
germination and the growth of thermo-resistant Bacillus and Lactobacillus may mask the presence of Listeria spp.
In wet weather, the spots spread and are covered by a gray, moldy, sporal
growth of the fungus.
regeneration of bracken (Pteridium aquilinium (L.) Kuhn.) in Finland in light of the dimensions and the age of its clones.
Quelques phases de l'ontogenese des cellules tapetales et sporales