Ovavesicula popillae reportedly produces 32 ovoid spores within a persistent sporophorous vesicle with a mean size of 20.0-21.0 [micro]m x 15.0-15.5 [micro]m (Andreadis & Hanula 1987).
popilliae infections were diagnosed by removing a small portion of Malpighian tubule from each beetle and examining them microscopically for sporophorous vesicles (Andreadis & Hanula 1987).
Beetle abdomens were macerated, and free sporophorous vesicles were examined under phase contrast microscopy at 400x magnification and measured with a calibrated ocular micrometer (n = 125).
Mean sporophorous vesicle size was 13.3 [micro]m (SE [+ or -] 0.1) x 9.3 [micro]m (SE [+ or -] 0.1) (Fig.
Ovavesicula popilliae's sporophorous vesicles are highly persistent and do not readily break when disturbed.
One explanation for the difference in size of our sporophorous vesicles and those originally described may be that our O.
However, on the basis of the feature of bisporous sporophorous
The parasite infects cells of the gut epithelium and initiates sporogony, which ends with the formation of a sporophorous vesicle (= SV), a spherical cluster of spores, surrounded by a membrane, inside the host cell.
The total number of parasite spores and sporophorous vesicles in a host can be quantified and their size measured using phase contrast microscopy.
This grinding method was used since counts of sporophorous vesicles are not possible from dead hosts.
After 15 days we dissected the gut of 41 of the newly infected females and counted the number of sporophorous vesicles in each infected gut cell.
Sporophorous vesicles (SVs) are clusters of spores produced by the parasite inside the infected gut cells.