is an approach to extracting three-dimensional information from a two-dimensional sample.
Early history of geometric probability and stereology
. Image Anal Stereol 31: 1-16.
Reed, Unbiased Stereology
: Three Dimensional Measurements in Microscopy, Springer, New York (1998).
For measurements, images were captured from an Olympus BH2 microscope using a video camera (Hitachi HV-C20, Tokyo, Japan) and were analyzed with Image Pro Plus software with a Stereology
5.0 plug-in (Media Cybernetics, Berkshire, UK).
To obtain an accurate estimate of the volume fraction of a particular tissue component, stereology
requires that the tissue of interest be sectioned in randomly oriented planes (Weibel, 1979).
: A Simple Comparison Between Two Semi-Automated Methods of Image Analysis for the Quantification of Collagen
The work was performed at Histomorphometry and Stereology
Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
applied to placental section allows minimal-biased and economical quantification of the three-dimensional structure of the placenta from molecular to whole-organ level .
Immunohistochemical staining of NOS isoforms, PAI-1, [alpha]-SMA, IL-4, IL-13, and bFGF-positive cells in alveolar septa, as well as endothelial, myofibroblast, and smooth muscle cells in terminal bronchiolar arteries, were quantified by stereology
at 400 x magnification with an eyepiece systematic point-sampling grid with 100 points and 50 lines used to count the fraction of lines overlying the positively stained structures (27).
for biologists and neuroscientists.
This year Image Analysis & Stereology
(IAS), the successor of Acta Stereologica celebrates 30 years of its existence.