The nerve-evoked contractions, excitatory junctional potentials (EJPs), and input resistance were measured using the gastric mill 4 (gm4) muscle (nomenclature of Maynard and Dando, 1974), one of the largest muscles in the stomatogastric system.
The stomatogastric nervous system (STNS) was dissected from the animal in physiological saline, fixed for 2-3 h in a solution of 4% paraformaldehyde (Sigma) in phosphate-buffered saline (PBS) at pH 7.0, and rinsed three times and incubated for 4 h in PBS containing 0.3% Triton X-100 (PBT).
In the American lobster Homarus americanus, exogenous application of Val-SIFamide to the isolated stomatogastric
ganglion produced a profound modulation of the output of the pyloric neural circuit (Christie et al., 2006).
Serotonergic innervation and modulation of the stomatogastric
ganglion of three decapod crustaceans (Panulirus interruptus, Homarus americanus and Cancer irroratus).
Ionic currents of the lateral pyloric neuron of the stomatogastric
ganglion of the crab.
Histamine as a neurotransmitter in the stomatogastric
nervous system of the spiny lobster.
Abbreviations: IBI, interburst interval; pSMN, putative swim motoneuron; sCPC, swim central pattern generator; STG, stomatogastric
Neurons of the decapod stomatogastric
ganglion display an important variant of the intrinsic properties seen in the DPs of the CG neurons.
The crab stomatogastric
nervous system (STNS) is an ideal preparation in which to address some of these questions.
An important recent finding is that CCAP is a potent modulator of the pyloric rhythm of the stomatogastric
ganglion of the crab Cancer borealis (Weimann et al., 1997).
In the pyloric network of the stomatogastric
ganglion of the lobster Panulirus interruptus, the qualitative pattern of functional connections between identified neurons has been shown to depend critically upon the modulatory neurohormonal environment of the circuit (Johnson et al., 1995).
Both have since been reported in the stomatogastric
system of the rock crab Cancer borealis (Weimann et al., 1993).