reported that noncovalent insertion of homobifunctional spacer Bissulfosuccinimidyl suberate
(BS3) to PLGA NPs during the NP preparation can efficiently facilitate the covalent amide bond between the antibody and NP surface.
(DSS) was purchased Thermo Fisher Scientific (Waltham, MA, USA).
The chips were then treated with cross-linker BS3 (bis[sulfosuccinimidyl] suberate
) (Thermo Scientific) 1.6 mM in PBS 1x pH = 7.4 at 4[degrees]C for 5h, washed three times with PBS 1x and once with deionized water.
The resuspended pellets were crosslinked with 4 mM fresh disuccinimidyl suberate
(DSS) cross-linker (Sigma) for 30 minutes at 37[degrees]C with flipping the tubes every 15 min and then pelleted by centrifugation at 6000 rpm for 10 min.
Given the large changes in Oat2 expression, the inhibitory potential of metabolites (adipate, suberate
, glycolate, citrate, 3-hydroxyisobutyrate, cis-aconitate, and homovanillate) associated with diabetic kidney disease [26, 27], drugs for treatment of diabetes (sitagliptin, miglitol), and hypertension (captopril, enalapril, furosemide, and bumetanide) on human OAT2 expressed in HEK293 cells was investigated.
To check the dimerization of our recombinant proteins, we used the cross-linking agents bissulfosuccinimidyl suberate
(BS3) and glutaraldehyde.
A synthetic 59-base oligonucleotide containing a C12 modifier was activated with excess disuccidimidyl suberate
Examples of such renewable dicarboxylic acids are dimethyl suberate
, dimethyl sebacate, or dimerized (un)saturated fatty acids.
Zinc dimerate dissolved in amines such as pyridine (9) while cadmium dicarboxylates (i.e., suberate
, sebacate, dodecanedioate, and terephthalate) dissolved in pyridine to form complexes (36) similar to those reported by Maritz (37) for copper, zinc, cadmium, and nickel adipates.
AZXMP was coupled to HRP by use of the homobifunctional reagent disuccinimidyl suberate
(DSS), which incorporates an 8-carbon chain linker.
Among these inborn errors are glutaric aciduria type I (18,19) (glutarate concentrations may be within reference values, whereas 3-hydroxyglutarate is present); medium-chain acyl-CoA dehydrogenase deficiency (adipate, suberate
, and sebacate concentrations may be within reference values, but the presence of suberylglycine and hexanoylglycine will reveal the disorder) (20); multiple acyl-CoA dehydrogenase deficiency, particularly in its mild forms (metabolites suggesting such a disease, including ethylmalonate and glutarate, are quite variable); and 2-ketoglutarate dehydrogenase deficiency (2-ketoglutarate excretion ranges from within reference values to 10 times higher than the upper limit of the reference interval).
For the cross-linking experiments, bis-sulfosuccinimidyl suberate
(BS3) was added as a cross-linker to the serum sample before immunoprecipitation.