Profacgen utilizes various methods for protein biotinylation including: Primary amine biotinylation, and Sulfhydryl
(thiol) content was quantified spectrophotometrically after derivatization by Ellmans reagent 5,5'-Dithiobis (2-nitrobenzoic acid) (DTNB) as described by Jongberg et al .
Serum free sulfhydryl
status is associated with patient and graft survival in renal transplant recipients.
Increased industrial activity, rapid urbanisation and agricultural requirement of chemicals and fertilisers have led to water pollution.3 The amount of As or its metabolites in biological materials such as hair, blood, nails and urine are used as biomarkers of As association.4 After absorption, As accumulates in biological samples such as in hair and nails, and it is thought to be involved in the binding of As to the sulfhydryl
groups in keratin.
Once therapeutic ozone is administrated, ozone dissolves in the plasma and responds with polyunsaturated fatty acids and sulfhydryl
It is conceivable that plasma GSH levels and GST or GPx activities in fish may be affected by 2,4-D and carbaryl due to that TAS levels reflect antioxidant molecules containing sulfhydryl
groups (Erel, 2004).
Arsenic renders its toxic effects by interacting with sulfhydryl
groups present in enzymes and interrupt enzymes concerning cellular respiration, which in turn is responsible for blocking glycolysis and Krebs cycle and replace phosphorus in a couple of biological processes6.
Enzyme systems, ischemia-modified albumin, protein carbonyl content, protein sulfhydryl
, malondialdehyde, lipid peroxides, and total antioxidant capacity were measured to understand the impact at tissue level.
The DTNB 5,5'-Dithiobis (2-nitrobenzoic acid) disulfide present in the measurement medium is a chromogen and is easily reduced by the sulfhydryl
In 1961, Frenkel and Hirsch described strains of streptococci isolated from cases of bacterial endocarditis that grew only in the presence of other bacteria, around which they formed satellite colonies, or in media enriched with sulfhydryl
compounds, such as cysteine.
(12) Moreover, CAP has been considered a free-radical scavenger due to its terminal sulfhydryl
Also, 4-HNE is a strong electrophile that can change cellular redox state significantly by decreasing cellular sulfhydryl
compounds such as glutathione (GSH).