Secretion of the methoxyindoles melatonin, 5-methoxytryptophol, 5-methoxyindoleacetic acid, and 5-methoxytryptamine from trout pineal organs in superfusion
culture: effects of light intensity.
In one study, rat brain slices exposed to an acute superfusion
of ethanol increased presynaptic GABA release and enhanced postsynaptic GABA receptor function in CeA neurons.
To construct the current versus voltage (I x V) plots, voltage ramps from -100 to +40 mV (500 ms long) were applied 800 ms after the beginning of the ATP superfusion
. This delay is necessary to get the current response at its peak and was extended for another 100 ms after the ramp was terminated.
, we found that superfusion
of euploid control-derived hippocampal slices with A[beta] oligomers (500 nM) produced a noticeable inhibition of 4xHFS-induced LTP (Figures 5(a) and 5(c)) in relation to untreated slices from these same animals.
RC-25; Warner Instruments, Hamden, CT, USA) attached to the stage of an upright microscope (Nikon Eclipse 80i; Nikon Corp., Tokyo, Japan) and continuously superfused (3 ml/min) with KRB applied to the recording chamber by gravity-fed superfusion
. Fluo-4 was excited at 488 nm with monochromatic light from an argon laser (Laser Physics, Reliant 100 s488, West Jordan, UT), coupled to a Yokogawa spin-disk confocal scan head (CSU10B, Yokogawa Electronic Co., Tokyo, Japan and Solamere Technology Group, Salt Lake city, USA).
Banaszak Holl, "Detergent induction of HEK 293A cell membrane permeability measured under quiescent and superfusion
conditions using whole cell patch clamp," The Journal of Physical Chemistry B, vol.
[sub.free][[[Ca.sup.2+]].sub.i] was determined 1.5-5 h after IR at 37[degrees]C during superfusion
with NaCl ringer (see above), upon [Ca.sup.2+] depletion with [Ca.sup.2+]-free NaCl ringer solution (in mM: 125 NaCl, 32 HEPES, 5 KCl, 5 D-glucose, 1 Mg[Cl.sub.2], and 0.5 EGTA, titrated with NaOH to pH 7.4), and during [Ca.sup.2+] readdition in NaCl ringer solution additionally containing ACA (0 or 20 [micro]M).
under aeration with carbogen ([O.sub.2]/C[O.sub.2]-95/5%), transferred into superfusion
chambers and rinsed with a calcium-free medium.
Thereafter, a depolarizing concentration of [K.sup.+] (KCl 28 mM) was introduced by addition in the superfusion
system for a 6 min period.
Granted specifically for Karmed's "Superfusion
" line of spinal implants, which focuses on interbody cages for therapeutic use in the cervical and lumbar areas, all implants in this product line are now produced using Peek-Optima HA Enhanced.
The compact SAR 7 flashlight weighs only 2.1 ounces and yet the SuperFusion
technology allows this 130-lumen LED light to project to 137 meters.