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It is established on the basis of decline of tetrazolium salt MTT (3-(4,5-dimethylthiazol-yl)-2,5-diphenyl tetrazolium bromide) by various dehydrogenase enzymes present in living cells.
The tetrazolium salt of WST-8 [2-(2-methoxy-4-nitrophenyl) -3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] was used as a metabolism indicator, as that salt penetrates into the viable cells, where it is metabolized in the mitochondria.
Tetrazolium test was performed with a sample of 110 pure seeds for each treatment, which were preconditioned for 24 hours in water and then sectioned using a razor blade.
To examine the injury caused by cerebral ischemia, we measured motor coordination and the infarct area using the rotarod test and triphenyl tetrazolium chloride staining, respectively.
Tetrazolium test: performed in four replicates of 25 embryos.
Nitroblue tetrazolium assay: Nitroblue tetrazolium (NBT) assay was determined by the method of Secombes (1990) as modified by Stasiack and Baumann (1996).
After harvest and 30 days after harvest the clean seeds of varieties from various sowing dates were tested for determing the germination %, seed viability (%) with Tetrazolium test, seed vigour (%) with Accelerated ageing test and Electrical Conductivity.
The microculture tetrazolium method was used to analyze the inhibition of the cell growth rate.
The enzyme mixture with 20 [micro]L of volume was mixed with 100 [micro]L of 2% (w/v) acetylated birchwood xylan and incubated at 40[degrees]C for 30 min, following which 1 ml of Tetrazolium Blue solution [0.
A kinetic colorimetric assay procedure is described for measuring lactate dehydrogenase activity, in which the reduction of NAD is coupled to the reduction of tetrazolium salt, 2-p-iodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride (INT), with phenazine methosulfate serving as an immediate electron carrier.
To study the serum alpha-1 antitrypsin levels in patients of CRF on long-term dialysis at different intervals (before, immediately after, and 8 hours after dialysis) and to appreciate and establish the extent of neutrophil activation in CRF patients by the Nitroblue Tetrazolium Test (NBT test).
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H- tetrazolium bromide assay on human umbilical vein endothelial cell proliferation
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