This is an observation encountered in tissues that are fixed in glutaraldehyde and postfixed in osmium tetroxide
. These neuroendocrine cells appeared primitive with sparse RER and rudimentary intracellular junctions more consistent with a neoplastic origin than entrapment or influx of normal cells.
For transmission electron microscopy (TEM), small pieces of testis with mature spermatozoa were fixed for 1 h with 2% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4), and postfixed with 1% osmium tetroxide
in the same buffer for 1 h.
Frog sartorious muscles were fixed with glutaraldehyde and osmium tetroxide
solutions and embedded in resin, then sliced into thin sections and mounted in a matching index medium (Euparal) on a strain-free microscope slide.
After a brief rinse in buffer, the tissue was postfixed in 2% osmium tetroxide
for 1 h, washed in buffer, dehydrated in ethanol, cleared in propylene oxide, and embedded in plastic.
Approximately 2,600 pounds of high purity hydrazine was supplied for the rocket launch and payload and 755 pounds of dinitrogen tetroxide
for the payload to deploy the fifth U.S.
Just before the SuperDraco thrusters were ignited, a "slug" of nitrogen tetroxide
(NTO) oxidizer was sent into a helium check valve made of titanium, which caused the valve to ignite, leading to the vehicle's explosion.
Today, the Yazoo City nitrogen manufacturing facility is located on approximately 2,240 acres and includes one ammonia plant, four nitric acid plants, an ammonium nitrate (AN) plant, two urea plants, a UAN plant, and a tetroxide
production and storage facility.
For the SEM analysis, the eggs were removed from the filter paper using a brush, fixed in 2.5% glutaraldehyde, and post-fixed in 1% osmium tetroxide
, both in 0.1 M sodium cacodylate buffer at 7.2 pH.
Thin slices of lung tissue fixed in formalin can be postfixed and stained in osmium tetroxide
solution and subsequently submitted for paraffin embedding.
The samples were cut into 3 [mm.sup.*] 3 [mm.sup.*] 5 mm and fixed with 3% glutaraldehyde overnight, rinsed 3 times in 0.1 M phosphate buffer (pH 7.2), and postfixed in 1% of osmium tetroxide
at 4[degrees]C for 2 h before being dehydrated with graded ethanol.
Singlet oxygen might also react with methionine persulfoxide 2 to form ozone and methionine sulfoxide 3 via tetroxide
intermediate 5 (Scheme 1).