Near-infrared spectra of powder blends were recorded using a Fourier-transform NIRS analyzer (Antaris II, ThermoElectron
Scientific, USA) in reflectance sampling configuration, equipped with an indium gallium arsenide (InGaAs) detector.
Each of the diluted samples was put in a quartz cell, which was placed in a Thermoelectron
UV-vis spectrophotometer (Genesis 10 UV), to measure the absorbance at the wavelength of 346 nm.
Copper concentrations were determined following USEPA Method 6010C (Inductively Coupled Plasma-Atomic Emission Spectrometry, USEPA 2007) using an inductively coupled plasma optical emission spectrophotometer (ThermoElectron
iCAP 6500 Inductively Coupled Plasma Optical Emission Spectrophotometer, Thermo Fisher Scientific, Inc., Waltham, MA).
Briefly, 25 L of reaction mixture containing 2.5 L of extracted DNA, 12.5 L of PCR master mix (2x) (Fermentas, Germany), 1 L of forward (10 pmoles/ L) and reverse (10 pmoles/ L) primers each (Oligos, GeneLink, USA), and 8 L of DEPC water was subjected to thermocycler (ThermoElectron
, Finland) under following conditions; initial denaturation at 94C for 4 min followed by 35 cycles, each of 94C for 30 seconds, annealing at 55C for 1 min and extension at 72C for 1 min.
Glucose and insulin concentrations were measured using standard assays (Thermoelectron
Infinity glucose reagent and Mercodia rat insulin ELISA, resp.).
The temperature of the specimen should not lead to the situation, when a significant thermoelectron
emission current is excited that is the noise for exoemission.
Plasma levels of TG and cholesterol were determined using Thermo Infinity TG and cholesterol reagents (ThermoElectron
, Melbourne, Australia).
The UV/V is spectra were recorded on a Thermospectronic Helios [gamma] UV (Thermoelectron
Co) spectrophotometer in a 1 cm quartz cuvette.
The steady-state anisotropy was measured in a He[gamma]10S[beta] spectrofluorophotometer (Thermoelectron
Corp., Sydney, Australia) equipped with a thermostated cell holder.
The eluates were analyzed by GC-MS using a ThermoElectron
Trace GC coupled to a DSQII quadrupole MS, all controlled by Xcalibur software.