Refolding of Misfolded Inclusion Bodies of Recombinant [alpha]-Amylase: Characterization of Cobalt Activated Thermostable
[alpha]-Amylase from Geobacillus SBS-4S.
vaccines have therefore been named a priority research area in the World Health Organisation's Global Vaccine Action Plan 2011-2020.
Industry requires thermostable
enzyme which should be stable for long time and can tolerate the harsh temperature conditions.
parahaemolyticus are associated with pathogenicity: thermostable
direct hemolysin (tdh) and thermostable
direct hemolysin-related hemolysin (trh) (Nishibuchi et al.
Current intention is to isolate strain of bacteria which can produce effective and functional phytases which are thermostable
at high temperature.
We have previously produced recombinant thermostable
phytase and now we have characterized the cellulase.
Subsequently, a genotype PCR assay for the MGL2-09 sample was performed by using 9 additional thermostable
DNA polymerases either with 3'-5' exonuclease activity ("proofreading") or without 3'-5' exonuclease activity ("nonproofreading"), including KAPA2G Robust HotStart (KAPA Biosystems), Klentaq1 (DNA Polymerase Technology, St Louis, Missouri), AmpliTaq Gold (Thermo Fisher Scientific), Platinum Taq (Thermo Fisher Scientific), FailSafe PCR Enzyme Blend (Lucigen Corp, Middleton, Wisconsin), TaKaRa LA Taq (Takara Bio USA, Mountain View, California), Platinum Taq High Fidelity (Thermo Fisher Scientific), Q5 High-Fidelity (New England Biolabs, Ipswich, Massachusetts), and Deep Vent DNA Polymerase (New England Biolabs).
The partners intend to develop thermostable
, solid-dose needle-free vaccines for a variety of infectious diseases and evaluate the potential to generate improved vaccine responses with simplified administration and reduced storage and distribution costs.
With better knowledge and purification of enzymes, there is an increase in number of applications, also the availability of thermostable
enzymes, aids in having a number of new possibilities for industrial processes have emerged (3).
In addition to being protective and thermostable
, RiVax has demonstrated that a reduced number of vaccinations may be required to establish protection, potentially utilizing only two doses instead of three.
formulation of RiVax has been shown to be stable for at least 12 months at temperatures up to 40 degrees celsius and to provide 100% protection to non-human primates exposed to aerosol ricin challenge in preclinical studies.Recent developments in the RiVax program have also described immune correlates of protection for the ricin toxin vaccine, which are important to facilitating potential approval of thermostabilized RiVax via the FDA "Animal Rule".