With the optimal reaction conditions in hand, we then synthesized the selected barbiturate and thiobarbiturate
derivatives by treating aldehydes [3-(p-methoxy) pyrazole aldehyde, 2-chloro quinoline aldehyde, Indole-3-carboxaldehyde and N,N-dimethyl benzaldehyde] with active methylene group containing compounds as barbituric acid, thiobarbituric acid and dimedone using1-butyl-3-methylimidazolium hydroxide (2 mL) as solvent medium.
Malondialdehyde is a component of thiobarbiturate
acid-reactive substances (TBARS) and the final product of lipid peroxidation.
Whereas, to establish the mechanistic pathway the same reaction was performed via stepwise methodology, initially synthesize of 5-benzylidene barbiturate and thiobarbiturate
derivatives were availed via Knoevenagel reaction  and their structure and purity was confirmed through spectroscopic techniques and eventually the Knoevenagel adducts were further reacted in the presence of sodium ethoxide as catalyst with equimolar amount of barbituric acid/thiobarbituric acid to avail highly functionalized tricyclic heterocycle compounds (1c-6c) and (1d-7d).
 in the nonsurvivors after paediatric ECMO found elevated levels of the lipid peroxidation markers: thiobarbiturate
acid-reactive substances (TBARS) and malondialdehyde.
Though IgE-mediated hypersensitivity reactions to thiopental, a thiobarbiturate
, have been described, no reports of IgE-mediated hypersensitivity reactions to methohexital, an oxybarbiturate, have been described .
Plasma was separated for the estimation of MDA by thiobarbiturate
(manual) method described by Jean CD et al.
The concentration of thiopental, a thiobarbiturate
, in the plasma of the first boy was 135 mg/L by an HPLC-ultraviolet detection method.
We used a slightly modified version of the colorimetric thiobarbiturate
assay by Warren (12).