"Confirmation and further
toxigenicity testing allowed for prompt case investigation and public health response, preventing disease spread."
difficile
toxigenicity founded in liquid stool samples of hospitalized patients and the possibility of asymptomatic carrying in 2% of patients with formed stool (17), the quantitative colony counts were sufficiently high to detect the bacterium irrespective of stool consistency and the semiformed stool should be sought for the pathogen in symptomatic patients with frequent stools18.
We performed
toxigenicity analysis and determined antidiphtheria toxin neutralization titers by Vero cell cytotoxicity assay, as described by Katsukawa et al.
Donner et al., "Distribution and
toxigenicity of Aspergillus species isolated from maize kernels from three agro-ecological zones in Nigeria," International Journal of Food Microbiology, vol.
A survey on distribution and
toxigenicity of Aspergillus section Flavi in poultry feeds.
[20.] Atehnkeng J, Ojiambo PS, Donner M, Ikotun T, Sikora RA and PJ Cotty Distribution and
toxigenicity of Aspergillus species isolated from maize kernels from three agro-ecological zones in Nigeria.
The isolates used were U0NV017 and U0NV003 and they had been tested for
toxigenicity through molecular characterization according to [3].
The fact that this discriminating test had been carried out and did not allow us to find any trace of toxin in our OTA- biocompetitor may be due to the its very weak
toxigenicity. It must be anyhow taken into account that mean OTA levels detected on some of the salami used for this study were lower than the threshold limit permitted by the Italian Regulation in swine meat and its derivatives (1 [micro]g/kg) [9], which so far represents the only existing guideline level for OTA in such products.
Levels and
Toxigenicity of Bacillus cereus and Clostridium perfringens from Retail Seafood, J.
Luczak, "Comparative study of thermos-resistance spores of Clostridium difficile strains belonging to different
toxigenicity groups," Medycyna doswiadczalna i mikrobiologia, vol.
In order to confirm the etiology of the outbreak as diphtheria, and because the Dominican Republic does not have
toxigenicity testing capability, 16 isolates were sent to the diphtheria laboratory at the Centers for Disease Control and Prevention (CDC; Atlanta, Georgia, United States) for real-time polymerase chain reaction (RT-PCR) confirmation.