The research team at ImmunoCellular successfully packaged a T cell receptor (TCR) DNA sequence into a lentiviral vector, which was then used to transfect
human hematopoietic stem cells.
Especially designed for primary cells and very difficult to transfect
Lipofectamine MessengerMAX is up to five times more efficient than DNA reagents, allowing researchers to easily transfect
biologically relevant cell models without electroporation or viruses.
Using lentiviral particles also allows effective direct visualization of Nuclei, Nuclear Membrane, Mitotic Chromosomes and Interphase Chromatin, Endosome, Endoplasmic Reticulum, Microtubule, Mitochondria, Golgi, Lysosome, Plasma Membrane, Cytoplasm and Peroxisome in hard to transfect
mammalian cells, stem cells and primary cells.
It is also not easy to transfect
oviduct cell due to unavailability of cell line.
By using the MaxCyte STX scalable transfection system, CCS Cell Culture Service is able to transfect
batches of up to 10 billion cells in [less than]30 min.
A key step in bringing this vector to the clinic was to demonstrate that it is both safe and feasible to transfect
the vector into human stem cells and for the RNA molecules to persist in the immune cells of the patient.
The Helios is designed to transfect
in vivo cells and has a pressure range of 100-600 psi.
The first experiment devoted to transfect
the male gamete was done in the rabbit by Brackett et al.
5 [micro]g/well) and 60 nmol of pre-miR negative control or pre-miR has-miR-206 (Ambion, USA) were premixed, and then the mixture was used to transfect
The hek 293 cells are very easy to work with, which means they can be straightforwardly grow in culture and to transfect
, and are very popular as hosts for gene expression.