transfect


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trans·fec·tion

 (trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.


trans·fect′ v.
American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. All rights reserved.

transfect

(trænsˈfɛkt)
vb
(Genetics) (tr) to bring about transfection in
[from trans- + (in)fect]
Collins English Dictionary – Complete and Unabridged, 12th Edition 2014 © HarperCollins Publishers 1991, 1994, 1998, 2000, 2003, 2006, 2007, 2009, 2011, 2014

transfect


Past participle: transfected
Gerund: transfecting

Imperative
transfect
transfect
Present
I transfect
you transfect
he/she/it transfects
we transfect
you transfect
they transfect
Preterite
I transfected
you transfected
he/she/it transfected
we transfected
you transfected
they transfected
Present Continuous
I am transfecting
you are transfecting
he/she/it is transfecting
we are transfecting
you are transfecting
they are transfecting
Present Perfect
I have transfected
you have transfected
he/she/it has transfected
we have transfected
you have transfected
they have transfected
Past Continuous
I was transfecting
you were transfecting
he/she/it was transfecting
we were transfecting
you were transfecting
they were transfecting
Past Perfect
I had transfected
you had transfected
he/she/it had transfected
we had transfected
you had transfected
they had transfected
Future
I will transfect
you will transfect
he/she/it will transfect
we will transfect
you will transfect
they will transfect
Future Perfect
I will have transfected
you will have transfected
he/she/it will have transfected
we will have transfected
you will have transfected
they will have transfected
Future Continuous
I will be transfecting
you will be transfecting
he/she/it will be transfecting
we will be transfecting
you will be transfecting
they will be transfecting
Present Perfect Continuous
I have been transfecting
you have been transfecting
he/she/it has been transfecting
we have been transfecting
you have been transfecting
they have been transfecting
Future Perfect Continuous
I will have been transfecting
you will have been transfecting
he/she/it will have been transfecting
we will have been transfecting
you will have been transfecting
they will have been transfecting
Past Perfect Continuous
I had been transfecting
you had been transfecting
he/she/it had been transfecting
we had been transfecting
you had been transfecting
they had been transfecting
Conditional
I would transfect
you would transfect
he/she/it would transfect
we would transfect
you would transfect
they would transfect
Past Conditional
I would have transfected
you would have transfected
he/she/it would have transfected
we would have transfected
you would have transfected
they would have transfected
Collins English Verb Tables © HarperCollins Publishers 2011
References in periodicals archive ?
Especially designed for primary cells and very difficult to transfect cell types.
LAPA scoring of 7 day differentiating cells revealed colonies from the TET2-pCDNA3 condition having an average LAPA score of 0.9 compared to 0.45 for the control group, showing that the TET2-pCDNA3 transfect colonies were on average 50% less differentiated than the control transfect colonies at the time of analysis (Figure 3(a)).
Electroporation also improves the researcher's ability to transfect for more difficult-to-transfect target cells, such as primary cells, and allows researchers to also transfect entire tissues in vivo.
In the present study, a lentivirus-mediated VEGF-shRNA system was used for the following reasons: lentiviruses efficiently transfect cells [41], lentiviral delivery of siRNA is suitable for local administration in ocular tissue, and the vector is transcribed in a DOX-dependent manner allowing easy control of shRNA transcription.
Calcium phosphate was used to transfect the endophilin2-pEGFPC1 (Endo2-GFP) construct and its control, or endophilin2 siRNA (Endo2 siRNA) and its control, into the neurons.
Using lentiviral particles also allows effective direct visualization of Nuclei, Nuclear Membrane, Mitotic Chromosomes and Interphase Chromatin, Endosome, Endoplasmic Reticulum, Microtubule, Mitochondria, Golgi, Lysosome, Plasma Membrane, Cytoplasm and Peroxisome in hard to transfect mammalian cells, stem cells and primary cells.
A key step in bringing this vector to the clinic was to demonstrate that it is both safe and feasible to transfect the vector into human stem cells and for the RNA molecules to persist in the immune cells of the patient.
The generated recombinant virus was collected and transfected into hFPPCs, followed by selection in 2 [micro]g/ml puromycin (Sigma) for 4 days.
SV40 large T antigen-transfected human leukemic Jurkat T cells (Jurkat-TAg) in logarithmic growth phase were transfected with plasmid DNAs by electroporation using NEPA21 Super Electroporator (NEPAGENE, Japan).
Human CL 1-0 cells stably transfected with PCNA expressing or control plasmids were treated with UV irradiation, H/A, and colcemid as described previously.
For MCU silencing, H9c2 cells were seeded at 2.5 x [10.sup.4] cells per well in 12-well plates and 24 h later were transfected with 18.8,112.5, or 225 nM of siRNA-MCU designed or siRNA-Neg using the HiPerFect[TM] Transfection Reagent (Qiagen[R], Venlo, Netherlands), according to the manufacturer's protocols.