transfect

(redirected from transfectants)

trans·fec·tion

 (trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.


trans·fect′ v.

transfect

(trænsˈfɛkt)
vb
(Genetics) (tr) to bring about transfection in
[from trans- + (in)fect]

transfect


Past participle: transfected
Gerund: transfecting

Imperative
transfect
transfect
Present
I transfect
you transfect
he/she/it transfects
we transfect
you transfect
they transfect
Preterite
I transfected
you transfected
he/she/it transfected
we transfected
you transfected
they transfected
Present Continuous
I am transfecting
you are transfecting
he/she/it is transfecting
we are transfecting
you are transfecting
they are transfecting
Present Perfect
I have transfected
you have transfected
he/she/it has transfected
we have transfected
you have transfected
they have transfected
Past Continuous
I was transfecting
you were transfecting
he/she/it was transfecting
we were transfecting
you were transfecting
they were transfecting
Past Perfect
I had transfected
you had transfected
he/she/it had transfected
we had transfected
you had transfected
they had transfected
Future
I will transfect
you will transfect
he/she/it will transfect
we will transfect
you will transfect
they will transfect
Future Perfect
I will have transfected
you will have transfected
he/she/it will have transfected
we will have transfected
you will have transfected
they will have transfected
Future Continuous
I will be transfecting
you will be transfecting
he/she/it will be transfecting
we will be transfecting
you will be transfecting
they will be transfecting
Present Perfect Continuous
I have been transfecting
you have been transfecting
he/she/it has been transfecting
we have been transfecting
you have been transfecting
they have been transfecting
Future Perfect Continuous
I will have been transfecting
you will have been transfecting
he/she/it will have been transfecting
we will have been transfecting
you will have been transfecting
they will have been transfecting
Past Perfect Continuous
I had been transfecting
you had been transfecting
he/she/it had been transfecting
we had been transfecting
you had been transfecting
they had been transfecting
Conditional
I would transfect
you would transfect
he/she/it would transfect
we would transfect
you would transfect
they would transfect
Past Conditional
I would have transfected
you would have transfected
he/she/it would have transfected
we would have transfected
you would have transfected
they would have transfected
References in periodicals archive ?
Stable transfectants were obtained after selection with G418 for different periods of time.
Luxembourg et al., "Probing the activation requirements for naive [CD.sup.+] T cells with Drosophila cell transfectants as antigen presenting cells," Immunological Reviews, vol.
Stable Keratinocyte Transfectants. HaCaT cells were stably transfected with myc/SOCS1, myc/SOCS2, myc/SOCS3, or empty pcDNA3 (mock) plasmids as previously reported [6, 13].
Victor Gonzales (Howard Hughes Medical Center, Dallas Texas) using enhanced GFP and isolation, by cell sorting of the stable transfectants. [15]
Twenty-four hours after transfection of pEGFP-C1-NS, stable transfectants were selected in gentamicin (G418; Life Technologies, USA) at a concentration of 800 [micro]g/ml.
Efficient selection for high-expression transfectants with a novel eukaryotic vector.
SLAT-transfected Jurkat-TAg cells showed an increase in TCR-induced adhesion to ICAM1 relative to control transfectants, as previously shown [16].
This strategy has many drawbacks [12]: (i) for a diploid asexual organism such as Leishmania, at least two rounds of transfection are required [13], and (ii) heterozygous transfectants need to be selected before the second round can be performed.
(35) Transfectants were selected by the 12-(1'-pyrene) dodecanoic acid (P12)/UV method, (36) showing peroxisomes as verified by staining with anti-catalase antibody (Fig.
After 48 h, transfectants were diluted in 1:10 ratio, and the antibiotic selection was performed in a medium containing 5 [micro]g/ml puromycin (Life Technologies, USA) for 4 weeks.
Fluorescence detection of green fluorescent protein indicated that both transfectants (vector only and the vector containing the MyoG sequence) had incorporated the plasmid.