were obtained after selection with G418 for different periods of time.
Luxembourg et al., "Probing the activation requirements for naive [CD.sup.+] T cells with Drosophila cell transfectants
as antigen presenting cells," Immunological Reviews, vol.
Stable Keratinocyte Transfectants
. HaCaT cells were stably transfected with myc/SOCS1, myc/SOCS2, myc/SOCS3, or empty pcDNA3 (mock) plasmids as previously reported [6, 13].
Victor Gonzales (Howard Hughes Medical Center, Dallas Texas) using enhanced GFP and isolation, by cell sorting of the stable transfectants
Twenty-four hours after transfection of pEGFP-C1-NS, stable transfectants
were selected in gentamicin (G418; Life Technologies, USA) at a concentration of 800 [micro]g/ml.
Efficient selection for high-expression transfectants
with a novel eukaryotic vector.
SLAT-transfected Jurkat-TAg cells showed an increase in TCR-induced adhesion to ICAM1 relative to control transfectants
, as previously shown .
This strategy has many drawbacks : (i) for a diploid asexual organism such as Leishmania, at least two rounds of transfection are required , and (ii) heterozygous transfectants
need to be selected before the second round can be performed.
were selected by the 12-(1'-pyrene) dodecanoic acid (P12)/UV method, (36) showing peroxisomes as verified by staining with anti-catalase antibody (Fig.
After 48 h, transfectants
were diluted in 1:10 ratio, and the antibiotic selection was performed in a medium containing 5 [micro]g/ml puromycin (Life Technologies, USA) for 4 weeks.
were prepared using a commercially available kit (Endofree Maxi-Prep; QIAGEN).
Fluorescence detection of green fluorescent protein indicated that both transfectants
(vector only and the vector containing the MyoG sequence) had incorporated the plasmid.