We are excited to have achieved this critical initial milestone in our Stem-to-T-Cell program, and to have generated a body of scientific evidence of successful transfection and proof that the transfected
human hematopoietic stem cells bearing the TCR were able to grow robustly for several generations," said Steven J.
micro ribonucleic acid 34-a mimics and antisense oligonucleotides into neuroblastoma cell line to explore its function in vitro through the variations.
U266 cells were transfected
with control or ALR shRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions.
Methods: HepG2 cells were stably transfected
with lentivirus-based short hairpin RNA vector targeting DcR3.
458: Stably Transfected
Human Androgen Receptor Transcriptional Activation Assay for Detection of Androgenic Agonist and Antagonist Activity of Chemicals
Generation of knockout pigs via SCNT and transfected
cells is associated with more advantages than generation of knockout pigs via DNA microinjection because the former method allows the assessment of vector integration in transfected
cells before they can be used as nuclear donors (Wolf et al.
They were then transfected
with 2 [micro]g plasmid vector or 100 pmol siRNA mixed with Lipofectamine 2000 reagent in serum reduced medium according to the manufacturer's instructions.
Moreover, an EGFP-C2-PiCacyBP eukaryotic expression plasmid was constructed and transfected
into 293T cells, which were grown under a series of salinity stresses.
To explore the assay behavior of wild-type human acid sphingomyelinase and the Q292K mutant, we transfected
HEK293 cells with a plasmid expressing either wild-type or mutant protein.
NIH-3T3 mouse fibroblast cell line was subsequently transfected
by the construct using Jet-PEI transfection reagent.
Human DCs matured with Ampligen(R) and transfected
with autologous tumour-specific mRNA are designed to elicit a potent and autologous tumoricidal antigen-specific cytotoxic response to the cancer.
Cell transfection and generation of cells stably transfected
with pGenesil-ERa small hairpin RNA (shRNA), pGenesil-ER[beta] shRNA and pGenesil-scrambled shRNA vectors