transfect

(redirected from transfected)

trans·fec·tion

 (trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.


trans·fect′ v.

transfect

(trænsˈfɛkt)
vb
(Genetics) (tr) to bring about transfection in
[from trans- + (in)fect]

transfect


Past participle: transfected
Gerund: transfecting

Imperative
transfect
transfect
Present
I transfect
you transfect
he/she/it transfects
we transfect
you transfect
they transfect
Preterite
I transfected
you transfected
he/she/it transfected
we transfected
you transfected
they transfected
Present Continuous
I am transfecting
you are transfecting
he/she/it is transfecting
we are transfecting
you are transfecting
they are transfecting
Present Perfect
I have transfected
you have transfected
he/she/it has transfected
we have transfected
you have transfected
they have transfected
Past Continuous
I was transfecting
you were transfecting
he/she/it was transfecting
we were transfecting
you were transfecting
they were transfecting
Past Perfect
I had transfected
you had transfected
he/she/it had transfected
we had transfected
you had transfected
they had transfected
Future
I will transfect
you will transfect
he/she/it will transfect
we will transfect
you will transfect
they will transfect
Future Perfect
I will have transfected
you will have transfected
he/she/it will have transfected
we will have transfected
you will have transfected
they will have transfected
Future Continuous
I will be transfecting
you will be transfecting
he/she/it will be transfecting
we will be transfecting
you will be transfecting
they will be transfecting
Present Perfect Continuous
I have been transfecting
you have been transfecting
he/she/it has been transfecting
we have been transfecting
you have been transfecting
they have been transfecting
Future Perfect Continuous
I will have been transfecting
you will have been transfecting
he/she/it will have been transfecting
we will have been transfecting
you will have been transfecting
they will have been transfecting
Past Perfect Continuous
I had been transfecting
you had been transfecting
he/she/it had been transfecting
we had been transfecting
you had been transfecting
they had been transfecting
Conditional
I would transfect
you would transfect
he/she/it would transfect
we would transfect
you would transfect
they would transfect
Past Conditional
I would have transfected
you would have transfected
he/she/it would have transfected
we would have transfected
you would have transfected
they would have transfected
References in periodicals archive ?
Transfected INS-1 cells were seeded in a 96-well plate with further culturing for 24 h.
M2 PHARMA-May 1, 2019-LineaRx Achieves Higher Expression and Survival Rates in Human T Cells Transfected Non-Virally with Linear DNA
The chicken macrophage (HD11) cells [19] were transiently transfected with a pcDNA3-eGFP vector containing either the LILRB1R-, LILRB1S-, LILRB3R-, or LILRB3S-encoding sequence and also mock control with empty pcDNA3-eGFP vector using Lipofectamine 3000 transfection reagent (Invitrogen, USA), as per the manufacturer's recommended protocols.
The scale of GFP expression was normalized using total cell numbers transfected with the same concentration of various constructs at the same conditions.
These cells were then transfected with pEGS/2SS-tPA, pEGS/2SS and pEGFP-N1 vector by using LipofectamineTM 2000 Kit (Invitrogen, Shanghai, China).
Cervical cancer cell lines were transfected with 50 nmol/L Small-interfering RNA (si-RNA) specifically targeted to NEAT1 (si- NEAT1 ) and negative control siRNA (si-NC) using lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).
Transfected GFP-LC3 plasmid in SW480 cells showed that PPAR[alpha] silence decreased autophagosome formation and GFP-LC3 puncta (Figure 1(b)), which was consistent with the transmission electron microscopy analysis (Figure 1(c)).
The cells in those seven groups were cultured in 6-well plates with 1 x [10.sup.5] cells per well, and several groups were transfected by the corresponding adenovirus as required.
Firstly, in order to observe functions of NUCB2 in bladder cancer, T24 and 5637 cells were transfected with specific shRNA to knockdown NUCB2.
Briefly, cells were transfected with 100 nM siCONT, siKRAS or siHRAS, and 0.3% Lipofectamine 2000 (Life Technologies, Mulgrave, Victoria, Australia), incubated for 24 h, and removed, and fresh medium was added to wells.
The following day, cells were transfected with mammalian expression plasmids of SLC15A3GFP or HA-SLC15A3 and control empty plasmids.