transfect

(redirected from transfecting)

trans·fec·tion

 (trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.


trans·fect′ v.
American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. All rights reserved.

transfect

(trænsˈfɛkt)
vb
(Genetics) (tr) to bring about transfection in
[from trans- + (in)fect]
Collins English Dictionary – Complete and Unabridged, 12th Edition 2014 © HarperCollins Publishers 1991, 1994, 1998, 2000, 2003, 2006, 2007, 2009, 2011, 2014

transfect


Past participle: transfected
Gerund: transfecting

Imperative
transfect
transfect
Present
I transfect
you transfect
he/she/it transfects
we transfect
you transfect
they transfect
Preterite
I transfected
you transfected
he/she/it transfected
we transfected
you transfected
they transfected
Present Continuous
I am transfecting
you are transfecting
he/she/it is transfecting
we are transfecting
you are transfecting
they are transfecting
Present Perfect
I have transfected
you have transfected
he/she/it has transfected
we have transfected
you have transfected
they have transfected
Past Continuous
I was transfecting
you were transfecting
he/she/it was transfecting
we were transfecting
you were transfecting
they were transfecting
Past Perfect
I had transfected
you had transfected
he/she/it had transfected
we had transfected
you had transfected
they had transfected
Future
I will transfect
you will transfect
he/she/it will transfect
we will transfect
you will transfect
they will transfect
Future Perfect
I will have transfected
you will have transfected
he/she/it will have transfected
we will have transfected
you will have transfected
they will have transfected
Future Continuous
I will be transfecting
you will be transfecting
he/she/it will be transfecting
we will be transfecting
you will be transfecting
they will be transfecting
Present Perfect Continuous
I have been transfecting
you have been transfecting
he/she/it has been transfecting
we have been transfecting
you have been transfecting
they have been transfecting
Future Perfect Continuous
I will have been transfecting
you will have been transfecting
he/she/it will have been transfecting
we will have been transfecting
you will have been transfecting
they will have been transfecting
Past Perfect Continuous
I had been transfecting
you had been transfecting
he/she/it had been transfecting
we had been transfecting
you had been transfecting
they had been transfecting
Conditional
I would transfect
you would transfect
he/she/it would transfect
we would transfect
you would transfect
they would transfect
Past Conditional
I would have transfected
you would have transfected
he/she/it would have transfected
we would have transfected
you would have transfected
they would have transfected
Collins English Verb Tables © HarperCollins Publishers 2011
References in periodicals archive ?
To determine whether LILRB1 and LILRB3 genes are associated with SHP-2, we examined their tyrosine phosphorylation profiles after transfecting LILRB1-eGFP and LILRB3-eGFP into the HD11 cell line as shown in Figure 4.
Square wave electroporation systems such as the BTX ECM 830 are ideal for transfecting mammalian cells and tissues, whereas exponential decay wave systems such as the BTX ECM 630 are ideal for transforming bacteria, yeast and other microorganisms.
NEAT1 was overexpressed in SiHa cell through transfecting with pcDNA-NEAT1 (pcDNA3.1 vector containing the whole sequence of NEAT1).
Like MYO-101, all programs rely upon transfecting a restorative gene utilizing the AAVrh.74 vector.
Under normal conditions, miR-448 was overexpressed in AGE1.HN and PC12 cells by transfecting miR-448 mimic, and apoptosis was significantly promoted (Figure 4B).
However, transfecting the same plasmid used by Yu and colleagues [10] was unsuccessful due to a dramatic decrease in cell viability.
After transfecting into adipocytes with different concentrations of TLR4 overexpression plasmids (4, 6, and 8 [micro]g/mL) for 24 h and 48 h, the TLR4 mRNA expression levels were significantly increased (P <0.01; Figure 2(a)); the highest efficiency was achieved with a concentration of 8 [micro]g/mL for 48 h (P < 0.001).
This was tested by transfecting H295R cells with miR-320a-3p Pre-miR and Anti-miR molecules and then measuring mRNA 48 hours posttransfection by qRT-PCR (Figure 5).
siRNA AF 488, Qiagen) has been used to monitor the efficiency of the chosen transfecting agent.
To validate this hypothesis, we further performed rescue experiments by transfecting si-NEAT1 or miR-129 mimic in combination with pcDNA-CTBP2 into EC109 cells.
The cDNA construct sequence was analyzed at the Penn State Hershey genomic Core Laboratories and the sequence confirmed using Blast alignment before transfecting the cells.
Liver hepatocellular cell (HepG2) represents a pure cell line of human liver carcinoma often used as hepatocellular carcinoma model due to the absence of viral infection and low level of NS2.10 This study aims at detecting the effects of HCV NS2 on oncogene expression including intracellular Bcl-2 and Bax by transfecting HepG2 cell with NS2 and C gene eukaryotic expression vectors so as to explore the role of NS2 in the occurrence of cirrhosis and cancer induced by HCV infection.