To determine whether LILRB1 and LILRB3 genes are associated with SHP-2, we examined their tyrosine phosphorylation profiles after transfecting
LILRB1-eGFP and LILRB3-eGFP into the HD11 cell line as shown in Figure 4.
Square wave electroporation systems such as the BTX ECM 830 are ideal for transfecting
mammalian cells and tissues, whereas exponential decay wave systems such as the BTX ECM 630 are ideal for transforming bacteria, yeast and other microorganisms.
NEAT1 was overexpressed in SiHa cell through transfecting
with pcDNA-NEAT1 (pcDNA3.1 vector containing the whole sequence of NEAT1).
Like MYO-101, all programs rely upon transfecting
a restorative gene utilizing the AAVrh.74 vector.
Under normal conditions, miR-448 was overexpressed in AGE1.HN and PC12 cells by transfecting
miR-448 mimic, and apoptosis was significantly promoted (Figure 4B).
the same plasmid used by Yu and colleagues  was unsuccessful due to a dramatic decrease in cell viability.
into adipocytes with different concentrations of TLR4 overexpression plasmids (4, 6, and 8 [micro]g/mL) for 24 h and 48 h, the TLR4 mRNA expression levels were significantly increased (P <0.01; Figure 2(a)); the highest efficiency was achieved with a concentration of 8 [micro]g/mL for 48 h (P < 0.001).
This was tested by transfecting
H295R cells with miR-320a-3p Pre-miR and Anti-miR molecules and then measuring mRNA 48 hours posttransfection by qRT-PCR (Figure 5).
siRNA AF 488, Qiagen) has been used to monitor the efficiency of the chosen transfecting
To validate this hypothesis, we further performed rescue experiments by transfecting
si-NEAT1 or miR-129 mimic in combination with pcDNA-CTBP2 into EC109 cells.
The cDNA construct sequence was analyzed at the Penn State Hershey genomic Core Laboratories and the sequence confirmed using Blast alignment before transfecting
Liver hepatocellular cell (HepG2) represents a pure cell line of human liver carcinoma often used as hepatocellular carcinoma model due to the absence of viral infection and low level of NS2.10 This study aims at detecting the effects of HCV NS2 on oncogene expression including intracellular Bcl-2 and Bax by transfecting
HepG2 cell with NS2 and C gene eukaryotic expression vectors so as to explore the role of NS2 in the occurrence of cirrhosis and cancer induced by HCV infection.