transfection


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trans·fec·tion

 (trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.


trans·fect′ v.

transfection

(trænsˈfɛkʃən)
n
(Genetics) the transfer into another cell of genetic material isolated from a cell or virus

trans•fec•tion

(trænsˈfɛk ʃən)

n.
the insertion into a bacterial cell of a viral nucleic acid in order to cause the cell to produce the virus.
[1964; trans- + (in) fection]
trans•fect′, v.t.
Translations
transfection
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References in periodicals archive ?
OncoSec will provide its proprietary Tavo and its new electroporation generator, Apollo, using lower voltage and a longer pulse width which greatly increased DNA-plasmid cellular transfection rates, to Duke University's Center for Applied Therapeutics.
Cell transfection was conducted using lipofetamine 3000 reagent (Invitrogen, USA) for 24 h.
Macherey-Nagel GmbH & Co KG said on Monday that it plans to develop an automated solution for the preparation of transfection grade plasmid DNA under a co-marketing agreement with Andrew Alliance.
* The target cells and tissues to which researchers may deliver CRISPR gene-editing tools will continue to expand by using more sophisticated transfection techniques such as electroporation.
It is also used as a transfection reagent as it has the capability to condense DNA to positively charged particles, which bind to negatively charged cell surface residues, and are introduced to a cell through endocytosis.
[USPRwire, Mon Oct 01 2018] According to the new market research report "Transfection Reagents and Equipment Market by Method (Biochemical (Calcium Phosphate, Lipofection, Dendrimers), Physical (Electroporation, Nucleofection), Viral)), Application (Biomedical, Protein Production), End User - Global Forecast to 2021" , analyzes and studies the major market drivers, restraints/challenges, and opportunities.
A transfection plasmid was inserted using FuGENE HD (Roche, Switzerland) according to the manufacturer's instructions.
TurboFect transfection reagent is a cationic polymer in water which forms compact, stable and positively charged complexes with DNA facilitating gene delivery into eukaryotic cells (www.thermofisher.com).
Particularly interesting is the interaction of lipophilic transition metal complexes and oligonucleotides and their ability to cross cellular compartments as indicated by their DNA transfection properties [9-11].
However, commonly used means of nonvirus carrier treatment, such as liposome-mediated transfection and electroporation, have a desirable effect in vitro but a reduced effect in vivo [4].