OncoSec will provide its proprietary Tavo and its new electroporation generator, Apollo, using lower voltage and a longer pulse width which greatly increased DNA-plasmid cellular transfection
rates, to Duke University's Center for Applied Therapeutics.
was conducted using lipofetamine 3000 reagent (Invitrogen, USA) for 24 h.
Macherey-Nagel GmbH & Co KG said on Monday that it plans to develop an automated solution for the preparation of transfection
grade plasmid DNA under a co-marketing agreement with Andrew Alliance.
* The target cells and tissues to which researchers may deliver CRISPR gene-editing tools will continue to expand by using more sophisticated transfection
techniques such as electroporation.
It is also used as a transfection
reagent as it has the capability to condense DNA to positively charged particles, which bind to negatively charged cell surface residues, and are introduced to a cell through endocytosis.
[USPRwire, Mon Oct 01 2018] According to the new market research report "Transfection
Reagents and Equipment Market by Method (Biochemical (Calcium Phosphate, Lipofection, Dendrimers), Physical (Electroporation, Nucleofection), Viral)), Application (Biomedical, Protein Production), End User - Global Forecast to 2021" , analyzes and studies the major market drivers, restraints/challenges, and opportunities.
plasmid was inserted using FuGENE HD (Roche, Switzerland) according to the manufacturer's instructions.
reagent is a cationic polymer in water which forms compact, stable and positively charged complexes with DNA facilitating gene delivery into eukaryotic cells (www.thermofisher.com).
Particularly interesting is the interaction of lipophilic transition metal complexes and oligonucleotides and their ability to cross cellular compartments as indicated by their DNA transfection
However, commonly used means of nonvirus carrier treatment, such as liposome-mediated transfection
and electroporation, have a desirable effect in vitro but a reduced effect in vivo .