transfect

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trans·fec·tion

 (trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.


trans·fect′ v.
American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. All rights reserved.

transfect

(trænsˈfɛkt)
vb
(Genetics) (tr) to bring about transfection in
[from trans- + (in)fect]
Collins English Dictionary – Complete and Unabridged, 12th Edition 2014 © HarperCollins Publishers 1991, 1994, 1998, 2000, 2003, 2006, 2007, 2009, 2011, 2014

transfect


Past participle: transfected
Gerund: transfecting

Imperative
transfect
transfect
Present
I transfect
you transfect
he/she/it transfects
we transfect
you transfect
they transfect
Preterite
I transfected
you transfected
he/she/it transfected
we transfected
you transfected
they transfected
Present Continuous
I am transfecting
you are transfecting
he/she/it is transfecting
we are transfecting
you are transfecting
they are transfecting
Present Perfect
I have transfected
you have transfected
he/she/it has transfected
we have transfected
you have transfected
they have transfected
Past Continuous
I was transfecting
you were transfecting
he/she/it was transfecting
we were transfecting
you were transfecting
they were transfecting
Past Perfect
I had transfected
you had transfected
he/she/it had transfected
we had transfected
you had transfected
they had transfected
Future
I will transfect
you will transfect
he/she/it will transfect
we will transfect
you will transfect
they will transfect
Future Perfect
I will have transfected
you will have transfected
he/she/it will have transfected
we will have transfected
you will have transfected
they will have transfected
Future Continuous
I will be transfecting
you will be transfecting
he/she/it will be transfecting
we will be transfecting
you will be transfecting
they will be transfecting
Present Perfect Continuous
I have been transfecting
you have been transfecting
he/she/it has been transfecting
we have been transfecting
you have been transfecting
they have been transfecting
Future Perfect Continuous
I will have been transfecting
you will have been transfecting
he/she/it will have been transfecting
we will have been transfecting
you will have been transfecting
they will have been transfecting
Past Perfect Continuous
I had been transfecting
you had been transfecting
he/she/it had been transfecting
we had been transfecting
you had been transfecting
they had been transfecting
Conditional
I would transfect
you would transfect
he/she/it would transfect
we would transfect
you would transfect
they would transfect
Past Conditional
I would have transfected
you would have transfected
he/she/it would have transfected
we would have transfected
you would have transfected
they would have transfected
Collins English Verb Tables © HarperCollins Publishers 2011
References in periodicals archive ?
Both transfections with miR-375 mimics and the miR-375 inhibitor up-regulated the target gene POU3F1.
(B) CCa cells with different transfections were treated with 5 [micro]M cisplatin for 24 h, followed by apoptosis ELISA assay.
All of the TransIT[R] transfection reagents can be used for reverse transfections with the following general recommendations based on the nucleic acid to be delivered.
Treatments and PPRE-H2B-eGFP and PPRE-pNL1.3[secNluc] Transfections. The cells were treated with 1 [micro]M GW1929 (agonist, #ab142213, Abcam) or 1 [micro]M GW9662 (antagonist, #ab141125, Abcam) diluted in a culture medium containing 1% glutamine and 10% FCS (without PS) for 2 h.
Nontreated (NT) cells served as a control throughout the transfections. hUCB-MSC were plated in a 96-well cluster dish at a density of 2 x [10.su.4] cells/mL, cultivated in DMEM containing 10% FBS.
Linear PEI 25 kDa (Polysiences Eppel-heim, Germany) was used as the transfection reagent for all transfections.
7-week-old adult (25-30 g) male Balb/c mice were used for in vivo transfections. Mice were anesthetized intramuscularly with xylazine (50 mg/kg) and ketamine (5-10 mg/kg).
In the 293 cells, on the second day after lacZ gene transfer, we found that X-gal positive cells were present in all receiving transfections expect only plasmid transfection (Figure 2).
All transfections were done using the calcium phosphate precipitation method of Berkner and Sharp (19).
The ability of the engineered fluorescent proteins to permeate cells obviates the need for transfections, allowing these novel proteins to be used in numerous biological applications.
Optimal transfections were achieved with 1 big DNA complexed with 18 nmol 22 kDa PEI 4 days after brain injections.
For the wild-type and mutated or deleted constructs, the copy number of human [beta]-globin mRNA per nanogram of total RNA from MEL cells was submitted to ANOVA with three trial factors (21) to assess the variability across PCR cycles (the first trial factor with three PCR cycles as levels), across the amount of calibrator RNA (the second trial factor with six levels), and across transfections (the third trial factor with three experiments as levels).