Among carbohydrate metabolizing enzymes, the activities of trehalase
, amylase and invertase were significantly reduced after treatment with sub lethal dose of esfenvalerate as compared to control.
Quantification of trehalose in biological samples with a conidial trehalose in biological samples with a conidial trehalase
from the thermophilic fungus Hudicolagrisea var.
gene was also under positive regulation of XYR1 .
Released glucose and trehalose-containing supernatants were added to 0.05 units of trehalase
. After 1 hr of enzymatic reaction at 37[degrees]C, the reaction suspension was mixed with water and 16% DNS reagent (3,5-dinitrosalicylic acid 1%, NaOH 2%, sodium potassium tartrate 20%) was added.
These components included carbohydrate, glycerol, soluble protein, esterase and trehalase
. Results showed that the total carbohydrate, glycogen and soluble protein contents were higher in the white cysts than in the brown cyst, whereas the glycerol content was lower in the white cysts than in the brown cysts.
It might be through an inhibition of acetic acid on the activity of disaccharide enzymes such as sucrase, maltase, trehalase
, and lactase in small intestine , as well as amylases [22, 25].
Phenylmethylsulfonyl fluoride (PMSF), ethylenediamine-tetraacetic acid (EDTA), 2,4-dinitrophenylhydrazine (DNPH), 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), 2,3,5-triphenyltetrazolium chloride (TTC), Girard's reagent T, amyloglucosidase (#10115), trehalase
(#T8778), peptone, and yeast extract were obtained from Sigma-Aldrich Corporation (USA); N,N,N',N'-tetramethylethylenediamine (TEMED) and quercetin were from Reanal (Hungary); disodium salt of alpha-ketoglutaric acid (99% purity) was from Protista AB (Sweden); all reagents for determination of glucose and triacylglycerides (TAG), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) activities were from PZ Cormay S.A.
Arguelles, "On the biochemical classification of yeast trehalases
: Candida albicans contains two enzymes with mixed features of neutral and acid trehalase
activities," Biochemical and Biophysical Research Communications, vol.
These include lactase deficiency (congenital and adult type), sucrase-isomaltase deficiency, maltase-glucoamylase deficiency, and trehalase
as a possible marker of intestinal ischemia – Reperfusion injury.
(1994), except that the trehalase
used in this assay was obtained from the fungus Rhizopus microsporus, following indications of Queiroz et al.
, invertase and amylase activities in the black scale Saissetia oleae, and their relation to host adaptability.