The three regimes subjected to evaluation are 1) New leaves + old leaves (FN + FV regime), collected in the V6--V8 stages, with five to seven fully opened trifoliolate
leaves; 2) New leaves + old leaves + pods at the beginning of grain development (FN + FV + LIG regime), which occurs at the R5.1 stage, thereby representing the beginning of grain formation; and 3) Old leaves + pods at the beginning of the development of the grain + pods with the well-developed grain (FV + LIG + LDG regime), which occurs in the stages R5.5--R6, characterized by legumes in full grain filling stage.
After sowing, all the plants were watered until the emergence of the trifoliolate
leaves after which watering was suspended on water-stressed plots.
A 0-5 scale was used, as follows: 0 = no wilt symptoms; 1 = wilt on one of the primary leaves; 2 = wilt on both primary leaves but not on the first trifoliolate
leaf; 3 = wilt on the first trifoliolate
leaf; 4 = death of the seedling after wilt development on the primary leaves and 5 = unmerged seedling or death of seedling before wilt development on the primary leaves.
Only fully opened trifoliolate
, tender leaves suitable for human consumption were hand-picked and their fresh weight was taken.
The plants were harvested after the appearance of 6-7 trifoliolate
leaves (between 6-8weeks after planting).
2010 Anthesis 01 May 2010 Beginning grain filling 20 May 2010 Physiological maturity Soybean 02 July 2010 21 g N, 16 g P, 21 kg K per OTC 06 July 2010 Emergence 11 July 2010 First trifoliolate
[20.] Ogunro Y Nutritional and toxicological studies on Dioscorea dumetorum (Trifoliolate
Pinnately compound trifoliolate
leaves are alternately attached to the stem, which terminates in a meristem.
I had assumed that my plant identification students, after a standard introductory leaf morphology practical session, would be able to consistently and confidently recognise and describe characters such as leaf arrangement (alternate, opposite of whorled), and leaf simple or compound (trifoliolate
, palmate, pinnate, bipinnate or tripinnate).
(1991) or from an unexpanded trifoliolate
leaf with a modified CTAB procedure of Keim et al.
Four pairs of trifoliolate
leaves were selected near the centre of each plant, comprising two pairs that showed no signs of disease and two pairs that demonstrated a low level of disease severity (the minimum level according to the diagrammatic scale proposed by GODOY et al., 2006).
A composite leaf tissue sample (approximately 50 mg) was collected from the first emerging trifoliolate
leaves from four plants of each RIL and the parents for both mapping populations.