Eosin Y, trypaflavine (Sigma, USA), and pyrene (Fluka, Germany) of the Purum brand were used as fluorescent probes.
The fluorescence of trypaflavine, eosin Y, and pyrene was measured within the spectral range of 475-575, 520-580, and 350-450 nm, respectively; the wavelength setting accuracy was 1 nm.
Two hydrophilic fluorescent dyes of different families, namely, trypaflavine (acriflavine chloride) and eosin Y, were used in our experiments .
Trypaflavine is an acridine dye, readily soluble in water.
Eosin Y fluorescence and trypaflavine fluorescence were studied in aqueous solutions before and after their dynamic sorption on fibers and in an adsorbed state.
The spectra of trypaflavine and eosin Y have one fluorescence maximum ([I.sub.max]) at wavelengths of 505 and 537 nm, respectively, in both aqueous phase and sorbent phase.
Table 2 shows that the extraction degree of trypaflavine and eosin Y on the CDA fiber is 24.2 and 3.3%, respectively.
Trypaflavine concentration on the fiber surface is supported by the appearance of its fluorescence signal on the matrix.
At similar extraction degrees of trypaflavine by the CTS fibers of both forms, the intensity of its signal in the adsorbed state on the polybasic CTS fiber was 3 times higher than on the polycationic CTS fiber.
CDA fibers are capable of sorbing trypaflavine on their surface; that is, they can be used as a matrix for SSF of this dye and similar (by chemical structure) probes.
For the CTS fibers it is shown that the SSF signal of eosin Y anions increases on the surface with the total positive charge (the polycationic form) and that of trypaflavine cations does on the surface with the total negative charge (the polybasic form); that is, the Coulomb interactions determine concentration and fluorescence of these probes on CTS matrices.
Selige, "Oxygen quenching of trypaflavine luminescence," Photochemistry and Photobiology, vol.