trypan blue


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trypan blue

(ˈtrɪpən; ˈtrɪpæn; trɪˈpæn)
n
(Biology) a dye obtained from tolidine that is absorbed by the macrophages of the reticuloendothelial system and is therefore used for staining cells in biological research
[so called because it is trypanocidal: see trypanosome, -cide]
Collins English Dictionary – Complete and Unabridged, 12th Edition 2014 © HarperCollins Publishers 1991, 1994, 1998, 2000, 2003, 2006, 2007, 2009, 2011, 2014
References in periodicals archive ?
Equal volumes of cell suspension and 0.4% trypan blue (Gibco) were mixed, 10uL of prepared sample were loaded in both chamber of hemocytometer.
Cell viability was assessed using trypan blue staining assay kit (Beyotime Biotechnology, China) and 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltertrazolium bromide tetrazolium (MTT) assay (Sigma-Aldrich).
Vital dyes, such as trypan blue (TB), can penetrate selectively into the dead cells, but the viable cells do not permit these dyes to penetrate the cells so the live cells remain unstained.
Cells number and viability were estimated using a haemocytometer under a light microscope after vital staining with trypan blue (Sigma-Aldrich, USA).
Methods: Possible cytotoxic, antiproliferative and anti-metastatic effects of the Allium extracts on MCF-7 and MDA-MB-231 cells were tested using trypan blue exclusion, MTT and wound heal assays, respectively.
After imaging, the viability of cells was determined using trypan blue exclusion assay as previously described (Strober, 2001; Archer et al, 2017; Freshney 2005).
Since the adipose stem cells in the nanofat were considered trophic factors, they contributed to tissue regeneration in the injected site.[6],[7] To analyze the cell viability, trypan blue staining was performed.
Both Trypan blue and MTT assays results confirm that FMSP-nanoparticles induced a dose-dependent cytotoxic effects on MCF-7 cells.
Dating back to the early 20th century, lots of blue dyes were synthesized like Methylene blue, Patent blue, Trypan blue, and so forth, and a comparison of main blue dyes was given in Table 1 [1-4].