Equal volumes of cell suspension and 0.4% trypan blue
(Gibco) were mixed, 10uL of prepared sample were loaded in both chamber of hemocytometer.
Cell viability was evaluated simultaneously using the Trypan Blue
Cell viability was assessed using trypan blue
staining assay kit (Beyotime Biotechnology, China) and 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltertrazolium bromide tetrazolium (MTT) assay (Sigma-Aldrich).
Viability of cells was determined using trypan blue
Vital dyes, such as trypan blue
(TB), can penetrate selectively into the dead cells, but the viable cells do not permit these dyes to penetrate the cells so the live cells remain unstained.
Cells number and viability were estimated using a haemocytometer under a light microscope after vital staining with trypan blue
Methods: Possible cytotoxic, antiproliferative and anti-metastatic effects of the Allium extracts on MCF-7 and MDA-MB-231 cells were tested using trypan blue
exclusion, MTT and wound heal assays, respectively.
The viability of the cells was measured after isolation by the trypan blue
exclusion assay and was found to be about 99 %.
After imaging, the viability of cells was determined using trypan blue
exclusion assay as previously described (Strober, 2001; Archer et al, 2017; Freshney 2005).
Since the adipose stem cells in the nanofat were considered trophic factors, they contributed to tissue regeneration in the injected site., To analyze the cell viability, trypan blue
staining was performed.
Both Trypan blue
and MTT assays results confirm that FMSP-nanoparticles induced a dose-dependent cytotoxic effects on MCF-7 cells.
Dating back to the early 20th century, lots of blue dyes were synthesized like Methylene blue, Patent blue, Trypan blue
, and so forth, and a comparison of main blue dyes was given in Table 1 [1-4].