iTRAQ([R]) utilizes isobaric tags to label peptides from a tryptic
digestion, followed by fractionation using a Nano HPLC column.
Seven different tryptic
peptides are monitored using multiple reaction monitoring (amino acid residues, peptide number): SV SEIQLMHNLGK (1-13, P1), LM HNLGK (7-13, P2), HLNSMER (14-20, P3), LQDVHNFVALGA PLAPR (28-44, P4), FVALGAPL APR (34-44, P5), VALGAPLAPR (35-44, P6), and ADVNVLTK (73-80, P7).
discovery-oriented proteomics research, primarily for the analysis of tryptic
digestion: The protein spots of interest were cut out from the preparative gels using pipette tips and extracted from the gels according to the published method (Dahlan et al.
The impact of tryptic
digestibility on amaranth-protein complexes has illustrated that dye may safely be used without any adverse effect.
Manual sample prep techniques involve 18 to 24 hr tryptic
digests prior to reverse-phase HPLC and mass spectrometry (MS).
The mutant strain exhibited slower growth in tryptic
soy broth (TSB) than the parental strain, but the two had similar growth rates in TSB that had been supplemented with yeast extract.
BACKGROUND: Preclinical results in rats have demonstrated anxiolytic-like effects of a tryptic
bovine alphaS1-casein hydrolysate.
americanum nymphs Protein in tryptic
digest Fed on rabbit Fed on sheep Immunoglobulin 3/4 3/4 Globin ([alpha] or [beta]) 1/4 3/4 Histone H3 1/4 3/4 Histone H2 3/4 3/4 Tubulin 1/4 2/4 Keratin 0/4 3/4 Actin 1/4 3/4 Cytochrome c-type heme lyase 0/4 0/4 [greater than or equal to] 1 of above 4/4 4/4 No.
DAT and tryptic
fragments were isolate by epitope specific immunoprecipitation and separated by SDS-PAGE analysis on a 9-16% gradient gel.
The most versatile kind of agar is the Tryptic
Soy agar used to determine the total count.
The SafeTsource product line also includes non-animal origin tryptic
soy broth culture media and a cryoprotectant agent.