These plates contained a 1.5% agar layer in water (5 mm) for leaf turgescence
(as adapted from BARBER et al., 1999).
The cuttings were collected in the morning to avoid dehydration, and were then stored in pails (buckets) with water for maintenance of turgescence
. No fungicide treatment was used so that the cuttings could express their rhizogenic potential.
On the other hand, this reduction observed in the RWC of the plants irrigated with water of elevated salt levels might be related to the increase in the damages caused in the cell membranes (Figure 1A), due to the increment in the leaching of ions which affected the cell water potential and, consequently, promoted a loss in the cell turgescence
. Thus, the decrease in the RWC might be an adaptation mechanism that, possibly, the plants developed, even allowing that, in low proportions, the absorption of water could occur (Garcia et al., 2009).
Topical nitroglycerine for intraoperative turgescence
. Anaesth Analg 1987;66(10):1022-3.
Furthermore, the jugular veins turgescence
highly suggestive for a cardiac disease, ECG documentation of atrial fibrillation and the left ventricular ejection fraction (LVEF) of 40 percent allowed the following diagnosis: dilated cardiomyopathy, permanent atrial fibrillation and chronic heart failure (NYHA II class).
They appear to be prone to substantial shrinkage: the turgescence
seems totally lost in dried specimens (Fig.